NGS in the clinical microbiology settings.

Autor:	Pitashny M; Clinical and Research Microbiome Center, Research Division, Rambam Health Care Campus, Haifa, Israel.; Clinical Microbiology Laboratories, Laboratories Division, Rambam Health Care Campus, Haifa, Israel., Kadry B; Clinical and Research Microbiome Center, Research Division, Rambam Health Care Campus, Haifa, Israel., Shalaginov R; Clinical Microbiology Laboratories, Laboratories Division, Rambam Health Care Campus, Haifa, Israel., Gazit L; Clinical Microbiology Laboratories, Laboratories Division, Rambam Health Care Campus, Haifa, Israel., Zohar Y; Pathology Institute, Rambam Health Care Campus, Haifa, Israel., Szwarcwort M; Clinical Microbiology Laboratories, Laboratories Division, Rambam Health Care Campus, Haifa, Israel., Stabholz Y; Infectious Diseases Unit, Rambam Health Care Campus, Haifa, Israel., Paul M; Infectious Diseases Unit, Rambam Health Care Campus, Haifa, Israel.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2022 Oct 19; Vol. 12, pp. 955481. Date of Electronic Publication: 2022 Oct 19 (Print Publication: 2022).
DOI:	10.3389/fcimb.2022.955481
Abstrakt:	We hypothesized that targeted NGS sequencing might have an advantage over Sanger sequencing, especially in polymicrobial infections. The study included 55 specimens from 51 patients. We compared targeted NGS to Sanger sequencing in clinical samples submitted for Sanger sequencing. The overall concordance rate was 58% (32/55) for NGS vs. Sanger. NGS identified 9 polymicrobial and 2 monomicrobial infections among 19 Sanger-negative samples and 8 polymicrobial infections in 11 samples where a 16S gene was identified by gel electrophoresis, but could not be mapped to an identified pathogen by Sanger. We estimated that NGS could have contributed to patient management in 6/18 evaluated patients and thus has an advantage over Sanger sequencing in certain polymicrobial infections. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Pitashny, Kadry, Shalaginov, Gazit, Zohar, Szwarcwort, Stabholz and Paul.)
Databáze:	MEDLINE
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