Dissection of a grain yield QTL from wild emmer wheat reveals sub-intervals associated with culm length and kernel number.

Autor: Deblieck M; Julius Kühn Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany., Szilagyi G; The Hebrew University of Jerusalem, Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot, Israel., Andrii F; University of Haifa, Institute of Evolution, Haifa, Israel., Saranga Y; The Hebrew University of Jerusalem, Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot, Israel., Lauterberg M; Research Group Genebank Documentation, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany., Neumann K; Research Group Genebank Documentation, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany., Krugman T; University of Haifa, Institute of Evolution, Haifa, Israel., Perovic D; Julius Kühn Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany., Pillen K; Department of Plant Breeding, Martin-Luther-University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences, Halle, Germany., Ordon F; Julius Kühn Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany.
Jazyk: angličtina
Zdroj: Frontiers in genetics [Front Genet] 2022 Oct 19; Vol. 13, pp. 955295. Date of Electronic Publication: 2022 Oct 19 (Print Publication: 2022).
DOI: 10.3389/fgene.2022.955295
Abstrakt: Genetic diversity in wheat has been depleted due to domestication and modern breeding. Wild relatives are a valuable source for improving drought tolerance in domesticated wheat. A QTL region on chromosome 2BS of wild emmer wheat ( Triticum turgidum ssp. dicoccoides ) , conferring high grain yield under well-watered and water-limited conditions, was transferred to the elite durum wheat cultivar Uzan ( T. turgidum ssp. durum ) by a marker-assisted backcross breeding approach. The 2B introgression line turned out to be higher yielding but also exhibited negative traits that likely result from trans-, cis-, or linkage drag effects from the wild emmer parent. In this study, the respective 2BS QTL was subjected to fine-mapping, and a set of 17 homozygote recombinants were phenotyped at BC 4 F 5 generation under water-limited and well-watered conditions at an experimental farm in Israel and at a high-throughput phenotyping platform (LemnaTec-129) in Germany. In general, both experimental setups allowed the identification of sub-QTL intervals related to culm length, kernel number, thousand kernel weight, and harvest index. Sub-QTLs for kernel number and harvest index were detected specifically under either drought stress or well-watered conditions, while QTLs for culm length and thousand-kernel weight were detected in both conditions. Although no direct QTL for grain yield was identified, plants with the sub-QTL for kernel number showed a higher grain yield than the recurrent durum cultivar Uzan under well-watered and mild drought stress conditions. We, therefore, suggest that this sub-QTL might be of interest for future breeding purposes.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Deblieck, Szilagyi, Andrii, Saranga, Lauterberg, Neumann, Krugman, Perovic, Pillen and Ordon.)
Databáze: MEDLINE
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