Functional changes in hemocytes and antioxidant activity in gills of the ark clam Anadara kagoshimensis (Bivalvia: Arcidae) induced by salinity fluctuations.

Autor: Kladchenko ES; Laboratory of ecological immunology of aquatic organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Lenninsky ave, 38, 119991 Moscow, Russia., Gostyukhina OL; Laboratory of ecological immunology of aquatic organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Lenninsky ave, 38, 119991 Moscow, Russia., Soldatov AA; Department of Animal Physiology and Biochemistry, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Lenninsky ave, 38, 119991 Moscow, Russia., Rychkova VN; Department of Animal Physiology and Biochemistry, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Lenninsky ave, 38, 119991 Moscow, Russia., Andreyeva AY; Laboratory of ecological immunology of aquatic organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Lenninsky ave, 38, 119991 Moscow, Russia. Electronic address: andreevaal@gmail.com.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2023 Feb-Mar; Vol. 264, pp. 110810. Date of Electronic Publication: 2022 Nov 04.
DOI: 10.1016/j.cbpb.2022.110810
Abstrakt: This study describes the analysis of antioxidant enzymatic activities (catalase and superoxide dismutase) in gills and functional state of hemocytes (osmotic stability, mitochondrial membrane potential) of ark clams (Anadara kagoshimensis) from the Black Sea basin exposed to salinity stress. For this, the effects of 48 h periods of exposure to low (8 ‰, 14 ‰) and high (35 ‰, 45 ‰) salinity were assessed. Our results showed that ark clams, A. kagoshimensis, possessed pronounced tolerance to hypersalinity stress and are sensitive to a short-time hyposalinity treatment. Salinity 35 ‰ inhibited production of reactive oxygen species (ROS) by hemocytes and did not affect their levels of mitochondrial membrane potential. Acclimation to 45 ‰ salinity caused significant increase in mitochondrial membrane potential accompanied with recovery of intracellular ROS levels up to controls levels. Acclimation to low salinity (8 ‰) induced an increase in both ROS and mitochondrial membrane potential levels in hemocytes. Catalase and superoxide dismutase activity in gills decreased following acclimation to low (8 ‰) and high (35 ‰) salinity. Exposure to the highest salinity levels (45 ‰) led to a decrease of superoxide dismutase activity levels, but did not influence the levels of catalase activity. Acclimation to low and high salinity was not accompanied with changes in osmotic fragility of hemocytes despite osmotic fragility curve according to changes in hemolymph osmolarity. Based upon these results, we postulate the involvement of cellular osmoregulatory mechanisms in the adaptation of the ark clam to short-term fluctuations of environmental salinity.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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