Wiring of bilirubin oxidases with redox polymers on gas diffusion electrodes for increased stability of self-powered biofuel cells-based glucose sensing.

Autor: Becker JM; Analytical Chemistry - Center for Electrochemical Sciences (CES), Faculty of Chemistry and Biochemistry, Ruhr-University Bochum, Universitätsstraße 150, D-44780 Bochum, Germany., Lielpetere A; Analytical Chemistry - Center for Electrochemical Sciences (CES), Faculty of Chemistry and Biochemistry, Ruhr-University Bochum, Universitätsstraße 150, D-44780 Bochum, Germany., Szczesny J; Analytical Chemistry - Center for Electrochemical Sciences (CES), Faculty of Chemistry and Biochemistry, Ruhr-University Bochum, Universitätsstraße 150, D-44780 Bochum, Germany., Bichon S; Centre de Recherche Paul Pascal, CNRS UMR 5031, University of Bordeaux, Avenue Albert Schweitzer, 33600 Pessac, France., Gounel S; Centre de Recherche Paul Pascal, CNRS UMR 5031, University of Bordeaux, Avenue Albert Schweitzer, 33600 Pessac, France., Mano N; Centre de Recherche Paul Pascal, CNRS UMR 5031, University of Bordeaux, Avenue Albert Schweitzer, 33600 Pessac, France., Schuhmann W; Analytical Chemistry - Center for Electrochemical Sciences (CES), Faculty of Chemistry and Biochemistry, Ruhr-University Bochum, Universitätsstraße 150, D-44780 Bochum, Germany. Electronic address: wolfgang.schuhmann@rub.de.
Jazyk: angličtina
Zdroj: Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2023 Feb; Vol. 149, pp. 108314. Date of Electronic Publication: 2022 Oct 27.
DOI: 10.1016/j.bioelechem.2022.108314
Abstrakt: A new redox polymer/bilirubin oxidase (BOD)-based gas diffusion electrode was designed to be implemented as the non-current and non-stability limiting biocathode in a glucose/O 2 biofuel cell that acts as a self-powered glucose biosensor. For the proof-of-concept, a bioanode comprising the Os-complex modified redox polymer P(VI-co-AA)-[Os(bpy) 2 Cl]Cl and FAD-dependent glucose dehydrogenase to oxidize the analyte was used. In order to develop an optimal O 2 -reducing biocathode for the biofuel cell Mv-BOD as well as Bp-BOD and Mo-BOD have been tested in gas diffusion electrodes in direct electron transfer as well as in mediated electron transfer immobilized in the Os-complex modified redox polymer P(VI-co-AA)-[Os(diCl-bpy) 2 ]Cl 2 . The resulting biofuel cell exhibits a glucose-dependent current and power output in the concentration region between 1 and 10 mM. To create a more realistic test environment, the performance and long-term stability of the biofuel cell-based self-powered glucose biosensor has been investigated in a flow-through cell design.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: ‘Wolfgang Schuhmann reports financial support was provided by European Commission.’
(Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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