Smartphone-based immunochemical sensor exploiting peroxidase-like activity of ligand-capped gold nanostars: A proof-of-concept detection of Mycobacterium bovis.

Autor: Lou-Franco J; Institute for Global Food Security, School of Biological Sciences, Queen's University of Belfast, 19 Chlorine Gardens, Belfast, BT9 5DL, UK., Zhao Y; Institute for Global Food Security, School of Biological Sciences, Queen's University of Belfast, 19 Chlorine Gardens, Belfast, BT9 5DL, UK; School of Electronics, Electrical Engineering and Computer Science, Queen's University Belfast, Stranmillis Road, Belfast, UK., Nelis JLD; Institute for Global Food Security, School of Biological Sciences, Queen's University of Belfast, 19 Chlorine Gardens, Belfast, BT9 5DL, UK; CSIRO Agriculture and Food, 306 Carmody Rd, St Lucia QLD, 4067, Australia., Stewart L; Institute for Global Food Security, School of Biological Sciences, Queen's University of Belfast, 19 Chlorine Gardens, Belfast, BT9 5DL, UK., Rafferty K; School of Electronics, Electrical Engineering and Computer Science, Queen's University Belfast, Stranmillis Road, Belfast, UK., Elliott C; Institute for Global Food Security, School of Biological Sciences, Queen's University of Belfast, 19 Chlorine Gardens, Belfast, BT9 5DL, UK; School of Food Science and Technology, Faculty of Science and Technology, Thammasat University, 99 Mhu 18, Pahonyothin Road, Khong Luang, Pathum Thani 12120, Thailand., Cao C; Institute for Global Food Security, School of Biological Sciences, Queen's University of Belfast, 19 Chlorine Gardens, Belfast, BT9 5DL, UK; Material and Advanced Technologies for Healthcare, Queen's University of Belfast, 18-30 Malone Road, Belfast, BT9 5BN, UK. Electronic address: c.cao@qub.ac.uk.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2023 Jan 15; Vol. 220, pp. 114857. Date of Electronic Publication: 2022 Oct 31.
DOI: 10.1016/j.bios.2022.114857
Abstrakt: Bacterial pathogens represent a safety concern in the food industry, and this is amplified by the lack of sensing devices that can be applied on-site by non-trained personnel. In this study, peroxidase-mimicking activity of gold nanostars was exploited to develop a user-friendly colourimetric sensor. A smartphone was exploited as an image reader and analyser, empowered with a novel App developed in-house. The mobile App was evaluated and compared with a commercial smartphone App for its capability to quantify generated colourimetric signals. A major obstacle found with sensors relying on gold nanozymes is the fact that modification of the surface of gold nanoparticles with biorecognition elements generally lead to a suppression of their nanozyme activity. This drawback was overcome by introducing an autocatalytic growth step, which successfully restored the peroxidase-mimicking activity through generation of new gold nanoseeds acting as catalytic centres. A proof-of-concept using this sensing mechanism was developed targeting Mycobacterium bovis, a zoonotic pathogen primarily found in cattle but that can be transmitted to humans by consumption of contaminated food and cause tuberculosis disease. The resulting smartphone-based immunological sensor has shown promising results with a linear response between 10 4 - 10 6  CFU/mL, enabling detection of M. bovis at concentrations as low as 7.2·10 3  CFU/mL in buffer conditions. It is anticipated that the concept of the developed approach will have applicability in many fields relying on smartphone-based biosensing.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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