Retinal regions shape human and murine Müller cell proteome profile and functionality.

Autor: Kaplan L; Department of Physiological Genomics, Ludwig-Maximilians-Universität München, Munich, Germany., Drexler C; Max Perutz Labs, Department of Biochemistry and Cell Biology, University of Vienna, Vienna Biocenter Campus (VBC), Vienna, Austria.; Vienna Biocenter PhD Program, Doctoral School of the University of Vienna and Medical University of Vienna, Vienna, Austria., Pfaller AM; Department of Physiological Genomics, Ludwig-Maximilians-Universität München, Munich, Germany., Brenna S; Neurology Department, Experimental Research in Stroke and Inflammation (ERSI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Wunderlich KA; Department of Physiological Genomics, Ludwig-Maximilians-Universität München, Munich, Germany., Dimitracopoulos A; Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK., Merl-Pham J; Research Unit Protein Science and Metabolomics and Proteomics Core, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany., Perez MT; Department of Clinical Sciences, Division of Ophthalmology, Lund University, Lund, Sweden.; NanoLund, Nanometer Structure Consortium, Lund University, Lund, Sweden., Schlötzer-Schrehardt U; Department of Ophthalmology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany., Enzmann V; Department of Ophthalmology, Bern University Hospital, Inselspital, University of Bern, Bern, Switzerland.; Department of BioMedical Research, University of Bern, Bern, Switzerland., Samardzija M; Department of Ophthalmology, University Hospital Zurich, University of Zurich, Zurich, Switzerland., Puig B; Neurology Department, Experimental Research in Stroke and Inflammation (ERSI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Fuchs P; Max Perutz Labs, Department of Biochemistry and Cell Biology, University of Vienna, Vienna Biocenter Campus (VBC), Vienna, Austria., Franze K; Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK.; Institute of Medical Physics, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.; Max-Planck-Zentrum für Physik und Medizin, Erlangen, Germany., Hauck SM; Research Unit Protein Science and Metabolomics and Proteomics Core, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany., Grosche A; Department of Physiological Genomics, Ludwig-Maximilians-Universität München, Munich, Germany.
Jazyk: angličtina
Zdroj: Glia [Glia] 2023 Feb; Vol. 71 (2), pp. 391-414. Date of Electronic Publication: 2022 Nov 05.
DOI: 10.1002/glia.24283
Abstrakt: The human macula is a highly specialized retinal region with pit-like morphology and rich in cones. How Müller cells, the principal glial cell type in the retina, are adapted to this environment is still poorly understood. We compared proteomic data from cone- and rod-rich retinae from human and mice and identified different expression profiles of cone- and rod-associated Müller cells that converged on pathways representing extracellular matrix and cell adhesion. In particular, epiplakin (EPPK1), which is thought to play a role in intermediate filament organization, was highly expressed in macular Müller cells. Furthermore, EPPK1 knockout in a human Müller cell-derived cell line led to a decrease in traction forces as well as to changes in cell size, shape, and filopodia characteristics. We here identified EPPK1 as a central molecular player in the region-specific architecture of the human retina, which likely enables specific functions under the immense mechanical loads in vivo.
(© 2022 The Authors. GLIA published by Wiley Periodicals LLC.)
Databáze: MEDLINE
Externí odkaz: