Protective effects of verbenalin and (+)-eudesmin against 6-hydroxydopamine-induced oxidative/nitrosative stress in SH-SY5Y cells.

Autor: Demiryürek AN; Gaziantep Sahinbey Municipality Science and Art Center, 27470, Gaziantep, Turkey., Göktürk Ö; Gaziantep Sahinbey Municipality Science and Art Center, 27470, Gaziantep, Turkey., Saracaloglu A; Department of Medical Pharmacology, Faculty of Medicine, Gaziantep University, 27310, Gaziantep, Turkey., Demiryürek S; Department of Physiology, Faculty of Medicine, Gaziantep University, 27310, Gaziantep, Turkey., Demiryürek AT; Department of Medical Pharmacology, Faculty of Medicine, Gaziantep University, 27310, Gaziantep, Turkey. demiryurek@gaziantep.edu.tr.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2023 Jan; Vol. 50 (1), pp. 331-338. Date of Electronic Publication: 2022 Nov 04.
DOI: 10.1007/s11033-022-08039-z
Abstrakt: Background: The purpose of this research was to study whether verbenalin, an iridoid glucoside, and (+)-eudesmin, a furofuran lignan isolated from different plant families, can attenuate cell damage and death induced by 6-hydroxydopamine (6-OHDA) in human neuroblastoma SH-SY5Y cells.
Methods: SH-SY5Y cells were incubated with 6-OHDA (35 µM) for 1 day. Verbenalin and (+)-eudesmin were administrated with various concentrations (1, 2.5, 5, 10, 20, and 50 µM) one hour before the 6-OHDA treatment. After 1 day, cell viability and neuroprotective effect were investigated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. Nitrosative stress was determined with measurements of nitric oxide (NO) and 3-nitrotyrosine (3-NT), a biomarker of peroxynitrite formation.
Results: We observed that 6-OHDA declined viability and augmented LDH leakage in SH-SY5Y cells. MTT analyses showed that pretreatment with verbenalin and (+)-eudesmin markedly prevented the toxicity due to 6-OHDA (P < 0.05). Verbenalin and (+)-eudesmin suppressed LDH release induced by 6-OHDA (P < 0.01). Although 6-OHDA treatment produced no marked effects on NO levels, (+)-eudesmin at high concentrations (10-50 µM) markedly attenuated NO levels (P < 0.01). There was a significant increase in 3-NT levels with 6-OHDA exposure in cells. Pretreatment with verbenalin, but not (+)-eudesmin, diminished 3-NT levels at low concentrations (1-20 µM) and prevented the cytotoxic effect of 6-OHDA (P < 0.01).
Conclusion: These results indicated that verbenalin and (+)-eudesmin exert potent cytoprotective activities against cytotoxicity triggered by 6-OHDA in neuroblastoma cells. This is the first report demonstrating that verbenalin may act as a peroxynitrite scavenger.
(© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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