Effect of Veau Class on Levator Veli Palatini Muscle Composition.
| Autor: | Chiang SN; Division of Plastic and Reconstructive Surgery, Department of Surgery, Washington University School of Medicine, St Louis, MO, USA., Meyer GA; Program in Physical Therapy, Washington University School of Medicine, St Louis, MO, USA.; Departments of Neurology, Orthopaedic Surgery, and Biomedical Engineering, Washington University School of Medicine, St Louis, MO, USA., Skolnick GB; Division of Plastic and Reconstructive Surgery, Department of Surgery, Washington University School of Medicine, St Louis, MO, USA., Hunter DA; Division of Plastic and Reconstructive Surgery, Department of Surgery, Washington University School of Medicine, St Louis, MO, USA., Wood MD; Division of Plastic and Reconstructive Surgery, Department of Surgery, Washington University School of Medicine, St Louis, MO, USA., Li X; Division of Plastic and Reconstructive Surgery, Department of Surgery, Washington University School of Medicine, St Louis, MO, USA., Snyder-Warwick AK; Division of Plastic and Reconstructive Surgery, Department of Surgery, Washington University School of Medicine, St Louis, MO, USA., Patel KB; Division of Plastic and Reconstructive Surgery, Department of Surgery, Washington University School of Medicine, St Louis, MO, USA. |
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| Jazyk: | angličtina |
| Zdroj: | The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association [Cleft Palate Craniofac J] 2024 Feb; Vol. 61 (2), pp. 319-325. Date of Electronic Publication: 2022 Nov 04. |
| DOI: | 10.1177/10556656221127840 |
| Abstrakt: | Objective: To examine levator veli palatini muscle composition in patients with nonsyndromic cleft palate and investigate the impact of Veau class. Design: Prospective cohort study. Setting: Tertiary care academic hospital. Patients/participants: Thirteen patients with nonsyndromic cleft palate were recruited. Interventions: During primary palatoplasty, a sample of levator veli palatini muscle was excised and prepared for histological analysis. Main Outcome Measures: Fat and collagen content were determined utilizing Oil Red and Sirius red stains, respectively, while muscle fiber cross-sectional areas were calculated from H&E-stained samples, with analysis using histomorphometric methods. Immunofluorescent staining of myosin heavy chain isoforms was performed. Results: Patients underwent repair at 10.8 months of age (interquartile range [IQR] 10.2-12.9). Fat content of the levator veli palatini muscle was low in both groups, ranging from 0% to 5.2%. Collagen content ranged from 8.5% to 39.8%; neither fat nor collagen content showed an association with Veau classes. Mean muscle fiber cross-sectional area decreased with increasing Veau class, from 808 µm 2 (range 692-995 µm 2 ) in Veau II to 651 µm 2 (range 232-750 µm 2 ) in Veau III ( P = .02). There was also a nonsignificant decrease in proportion of type I muscle fibers with increasing Veau class (44.3% [range 31.4%-84.4%] in Veau II vs 35.3% [range 17.4%-61.3%] in Veau III). Conclusions: Muscle fiber area in levator veli palatini muscles decreases in Veau III clefts in comparison to Veau II. The impact of these differences in velopharyngeal dysfunction requires further analysis of a larger cohort. Competing Interests: Declaration of Conflicting InterestsThe author(s) declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: KBP is a consultant for Stryker CMF. |
| Databáze: | MEDLINE |
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