[Eukaryotic expression of human Y-box binding protein 1 (YB-1) promotes the proliferation and migration of human HepG2 cells].

Autor:	Shi Y; Medical College, Guizhou University, Guiyang 550025; Department of Medical Molecular Biology, Institute of Biotechnology, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China., Ge X; Department of Medical Molecular Biology, Institute of Biotechnology, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850; Department of Oncology, the Second Medical Center, General Hospital of PLA, Beijing 100853, China., Jiang Q; Department of Medical Molecular Biology, Institute of Biotechnology, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China., Xie T; Department of Medical Molecular Biology, Institute of Biotechnology, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China., Mi Y; Department of Medical Molecular Biology, Institute of Biotechnology, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China., Song S; Jinzhou Medical University, Jinzhou 121000, China., Zhang D; Department of Medical Molecular Biology, Institute of Biotechnology, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China. *Corresponding authors, E-mail: ggatsby@126.com., Ye Q; Department of Medical Molecular Biology, Institute of Biotechnology, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China. *Corresponding authors, E-mail: yeqn66@yahoo.com.
Jazyk:	čínština
Zdroj:	Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2022 Nov; Vol. 38 (11), pp. 986-991.
Abstrakt:	Objective To establish the eukaryotic expression vector of Y-box-binding protein 1 (YB-1) with FLAG-tagged and transfect it into hepatocellular carcinoma HepG2 cells to identify the effects of YB-1 on the proliferation and migration. Methods Human YB-1 gene was amplified from the human ovary library by PCR. YB-1 fraction was double enzyme digested and connected with pcDNA3.0-FLAG vector to construct eukaryotic expression vector pcDNA3.0-FlAG-YB-1, which was transfected into HepG2 cells. The expression of YB-1 was detected by Western blotting, and the effect of YB-1 on the proliferation of HepG2 cells was determined by CCK-8 assay and clone formation. The effect of YB-1 on the migration of HepG2 cells was analyzed by wound healing assays. Results The eukaryotic expression vector pcDNA3.0-FLAG-YB-1 was successfully established. YB-1 protein can be expressed in HepG2 cells, and YB-1 promoted the proliferation and migration of HepG2 cells. Conclusion YB-1 promotes the proliferation and migration of HepG2 cells.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu