Oropharyngeal samples versus lesion specimens at diagnosis in patients infected with monkeypox virus in Northern France.

Autor: Ouafi M; Univ Lille, CHU Lille, Laboratoire de Virologie ULR3610, Lille, France., Regueme A; Univ Lille, CHU Lille, Laboratoire de Virologie ULR3610, Lille, France., Alcaraz I; CH Tourcoing, Service Universitaire de Maladies Infectieuses et Tropicales, Tourcoing, France., Riviere P; Département de Maladies Infectieuses, CHU Lille, Lille, France., Bazus H; CH Lens, Service de Maladies Infectieuses et Tropicales, Lens, France., Salmon-Rousseau A; Département de Maladies Infectieuses, CHU Lille, Lille, France., Cappeliez B; CH Tourcoing, Service Universitaire de Maladies Infectieuses et Tropicales, Tourcoing, France., Cartier N; Univ Lille, CHU Lille, Laboratoire de Virologie ULR3610, Lille, France., Guigon A; Univ Lille, CHU Lille, Laboratoire de Virologie ULR3610, Lille, France., Lazrek M; Univ Lille, CHU Lille, Laboratoire de Virologie ULR3610, Lille, France., Bocket L; Univ Lille, CHU Lille, Laboratoire de Virologie ULR3610, Lille, France., Faure E; Département de Maladies Infectieuses, CHU Lille, Lille, France., Robineau O; CH Tourcoing, Service Universitaire de Maladies Infectieuses et Tropicales, Tourcoing, France., Hober D; Univ Lille, CHU Lille, Laboratoire de Virologie ULR3610, Lille, France., Alidjinou EK; Univ Lille, CHU Lille, Laboratoire de Virologie ULR3610, Lille, France.
Jazyk: angličtina
Zdroj: Journal of medical virology [J Med Virol] 2023 Jan; Vol. 95 (1), pp. e28276. Date of Electronic Publication: 2022 Nov 09.
DOI: 10.1002/jmv.28276
Abstrakt: The ongoing outbreak of monkeypox virus (MPXV) is the largest one in historically non-endemic countries. Early reports described atypical epidemiological and clinical presentations. We investigated MPXV DNA detection in oropharyngeal samples (OPS), and compared the viral load to that in lesion samples at diagnosis in patients infected with MPXV. We retrospectively included patients suspected to have monkeypox in Northern France, who underwent a MPXV PCR in the Virology Laboratory, University Hospital of Lille, from May 23 to August 18, 2022. Overall, a total of 228 patients (376 samples) were included. A positive result in at least one sample was found in 138 patients (60.5%). We compared PCR results between OPS and lesion samples (i.e., cutaneous or anal/rectal samples) in patients with both samples. A positive result in OPS was observed in 54 out of 60 patients (90%). The viral load in OPS (median C t value = 29.5; interquartile range [IQR] = 24.7-34) was significantly lower than that in lesion samples (median C t  value = 17.8; IQR = 16.3 and 19.7) (p < 0.0001). This report shows that pharyngeal sampling does not bring additional information for the initial diagnosis in patients presenting with typical lesions.
(© 2022 Wiley Periodicals LLC.)
Databáze: MEDLINE
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