UiO-66-based metal-organic framework for dispersive solid-phase extraction of vanillylmandelic acid from urine before analysis by capillary electrophoresis.

Autor:	Hassanpour-Sabet R; Food and Drug Safety Research Center, Tabriz University of Medical Sciences Tabriz Iran mr.afsharmogaddam@yahoo.com afsharmogaddam@tbzmed.ac.ir +98 41 31773333 +98 41 33372250., Seyfinejad B; Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences Tabriz Iran ajouyban@hotmail.com.; Student Research Committee, Tabriz University of Medical Sciences Tabriz Iran., Marzi Khosrowshahi E; Food and Drug Safety Research Center, Tabriz University of Medical Sciences Tabriz Iran mr.afsharmogaddam@yahoo.com afsharmogaddam@tbzmed.ac.ir +98 41 31773333 +98 41 33372250., Nemati M; Food and Drug Safety Research Center, Tabriz University of Medical Sciences Tabriz Iran mr.afsharmogaddam@yahoo.com afsharmogaddam@tbzmed.ac.ir +98 41 31773333 +98 41 33372250., Afshar Mogaddam MR; Food and Drug Safety Research Center, Tabriz University of Medical Sciences Tabriz Iran mr.afsharmogaddam@yahoo.com afsharmogaddam@tbzmed.ac.ir +98 41 31773333 +98 41 33372250.; Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences Tabriz Iran ajouyban@hotmail.com., Jouyban A; Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences Tabriz Iran ajouyban@hotmail.com.; Faculty of Pharmacy, Near East University PO BOX: 99138 Nicosia Mersin 10 North Cyprus Turkey.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2022 Oct 10; Vol. 12 (44), pp. 28728-28737. Date of Electronic Publication: 2022 Oct 10 (Print Publication: 2022).
DOI:	10.1039/d2ra02916b
Abstrakt:	Dispersive solid-phase extraction (DSPE) was developed for the extraction of vanillylmandelic acid (VMA) in urine samples prior to capillary electrophoresis with diode array detection (CE-DAD). Extraction of VMA by DSPE was carried out by direct addition of 7.5 mg of synthesized amino-functionalized UiO-66 (Zr) metal-organic framework adsorbent into the 5 mL sample solution (pH 4.0), followed by sonication and centrifugation. The supernatant layer was discarded, then the sedimented adsorbent was eluted using borate buffer (75 mM, pH 10). Effective extraction parameters including the amount of adsorbent, sample pH, adsorption and desorption time, type, volume and pH of eluent, and type of adsorbent dispersion method were systematically investigated. Under the optimized conditions, linearity of the method was from 40 to 2000 μg L -1 with a correlation coefficient over 0.9948. The method detection and quantification limits were 12 and 40 μg L -1 , respectively. The relative standard deviations for intra-and inter-day precision were 2.4 and 2.8% ( n = 5), respectively. The extraction recovery and enrichment factor values were 90% and 9.0 respectively. Competing Interests: The authors declare that they have no conflict of interest. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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