A novel competitive ELISA for detection of antibodies against bovine viral diarrhea virus infection.

Autor: Seyfi Abad Shapouri MR; Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran., Mahmoodi P; Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran., Ghorbanpour Najafabadi M; Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran., Haji Haji-Kolaei MR; Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran., Moradi Choghakabodi P; Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran., Lotfi M; Quality Control Department, Razi Vaccine and Serum Research Institute, Agricultural Education and Extension Research Organization (AREEO), Karaj, Iran., Pourmahdi Boroujeni M; Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran., Ekhtelat M; Department of Pharmacognosy, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran., Daghari M; Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Jazyk: angličtina
Zdroj: Veterinary research forum : an international quarterly journal [Vet Res Forum] 2022 Sep; Vol. 13 (3), pp. 403-407. Date of Electronic Publication: 2022 Sep 15.
DOI: 10.30466/vrf.2021.521500.3135
Abstrakt: Diagnosis of bovine viral diarrhea (BVD) relies on the detection of antibodies against its viral causing agent, bovine viral diarrhea virus (BVDV). Here, we designed a novel competitive ELISA (cELISA) using the most immunogenic part of BVDV nonstructural protein 3 (NS 3 ), as a single ELISA recombinant antigen, along with a monoclonal antibody to detect antibodies against BVDV in sera of infected animals. Hence, 197 serum samples were tested by this cELISA and the results were compared to the results obtained from virus neutralization test (VNT) as the gold standard method for diagnosis of BVD. McNemar's test indicated that there was no significant difference between the results of this newly designed cELISA and VNT. Meanwhile, kappa coefficients showed that there was a high correlation between these two assays. The relative sensitivity and specificity of cELISA with respect to VNT were 93.90% and 100%, respectively, suggesting that this newly designed cELISA could be a useful diagnostic tool for detection of BVDV infection. Moreover, as NS 3 is highly conserved among Pestiviruses and the developed ELISA is a competitive one, it could potentially be applied to detect BVDV infection in other domestic and wildlife species.
Competing Interests: The authors declare no conflict of interest.
(© 2022 Urmia University.)
Databáze: MEDLINE
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