| Autor: |
Wu JC; College of Pharmacy, Mudanjiang Medical University, Mudanjiang 157400, China., Jin SD; College of Pharmacy, Harbin Medical University, Harbin 150081, China., Song JH; College of Pharmacy, Harbin Medical University, Harbin 150081, China., Liu XQ; College of Pharmacy, Harbin Medical University, Harbin 150081, China., Ma WJ; College of Pharmacy, Mudanjiang Medical University, Mudanjiang 157400, China., Chang L; College of Pharmacy, Mudanjiang Medical University, Mudanjiang 157400, China., Guan XX; College of Pharmacy, Harbin Medical University, Harbin 150081, China., Zhang MY; College of Pharmacy, Harbin Medical University, Harbin 150081, China., Liu JQ; College of Pharmacy, Mudanjiang Medical University, Mudanjiang 157400, China., Fu H; College of Pharmacy, Mudanjiang Medical University, Mudanjiang 157400, China., Wang Y; College of Basic Medicine, Mudanjiang Medical University, Mudanjiang 157400, China. wangxingliang78@163.com., Xu CQ; College of Pharmacy, Harbin Medical University, Harbin 150081, China. xuchaoqian@ems.hrbmu.edu.cn. |
| Abstrakt: |
The present study was aimed to investigate the effects of circRNA-0028171 on the apoptosis of vascular endothelial cells induced by arsenic trioxide (As 2 O 3 ). Human umbilical vein endothelial cells (HUVECs) were treated with 0-15 μmol/L As 2 O 3 for 24 h. Then, cellular viability was measured by MTT assay. The expression levels of circRNA-0028171, Bcl-2 and Bax mRNA were detected by real-time quantitative PCR. Bcl-2/Bax protein ratio was detected by Western blot. Whether circRNA-0028171 was involved in the regulation of HUVECs by As 2 O 3 was investigated by transfection with overexpression plasmid of circRNA-0028171 and siRNA. The results showed that compared with the control group, As 2 O 3 group showed decreased cellular viability, reduced Bcl-2/Bax mRNA and protein ratios, and significantly lower expression of circRNA-0028171. Overexpression of circRNA-0028171 inhibited apoptosis of HUVECs induced by As 2 O 3 . Knockdown of circRNA-0028171 by siRNA promoted As 2 O 3 -induced apoptosis in HUVECs. These results suggest that circRNA-0028171 is involved in the vascular endothelial cell apoptosis induced by As 2 O 3 . |
