Resonant Acoustic Rheometry to Measure Coagulation Kinetics in Hemophilia A and Healthy Plasma: A Novel Viscoelastic Method.

Autor:	Li W; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan., Hobson EC; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan., Bunch CM; Department of Emergency Medicine and Internal Medicine, Henry Ford Hospital, Detroit, Michigan., Miller JB; Department of Emergency Medicine and Internal Medicine, Henry Ford Hospital, Detroit, Michigan., Nehme J; Department of Emergency Medicine and Internal Medicine, Henry Ford Hospital, Detroit, Michigan., Kwaan HC; Division of Hematology and Oncology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois., Walsh MM; Department of Emergency Medicine, St. Joseph Regional Medical Center, Mishawaka, Indiana., McCurdy MT; Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland., Aversa JG; Department of Surgery, Indiana University School of Medicine, Indianapolis, Indiana., Thomas AV; Indiana University School of Medicine South Bend Campus, Notre Dame, Indiana., Zackariya N; Indiana University School of Medicine South Bend Campus, Notre Dame, Indiana., Thomas SJ; Department of Emergency Medicine, St. Joseph Regional Medical Center, Mishawaka, Indiana., Smith SA; Department of Biological Chemistry, Michigan Medicine, Ann Arbor, Michigan., Cook BC; Department of Pathology, Henry Ford Hospital, Detroit, Michigan., Boyd B; Department of Pathology, Henry Ford Hospital, Detroit, Michigan., Stegemann JP; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan., Deng CX; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan.
Jazyk:	angličtina
Zdroj:	Seminars in thrombosis and hemostasis [Semin Thromb Hemost] 2023 Mar; Vol. 49 (2), pp. 201-208. Date of Electronic Publication: 2022 Nov 01.
DOI:	10.1055/s-0042-1757896
Abstrakt:	Compared with conventional coagulation tests and factor-specific assays, viscoelastic hemostatic assays (VHAs) can provide a more thorough evaluation of clot formation and lysis but have several limitations including clot deformation. In this proof-of-concept study, we test a noncontact technique, termed resonant acoustic rheometry (RAR), for measuring the kinetics of human plasma coagulation. Specifically, RAR utilizes a dual-mode ultrasound technique to induce and detect surface oscillation of blood samples without direct physical contact and measures the resonant frequency of the surface oscillation over time, which is reflective of the viscoelasticity of the sample. Analysis of RAR results of normal plasma allowed defining a set of parameters for quantifying coagulation. RAR detected a flat-line tracing of resonant frequency in hemophilia A plasma that was corrected with the addition of tissue factor. Our RAR results captured the kinetics of plasma coagulation and the newly defined RAR parameters correlated with increasing tissue factor concentration in both healthy and hemophilia A plasma. These findings demonstrate the feasibility of RAR as a novel approach for VHA, providing the foundation for future studies to compare RAR parameters to conventional coagulation tests, factor-specific assays, and VHA parameters. Competing Interests: M.M.W. has received honoraria from Alexion Pharmaceuticals. (Thieme. All rights reserved.)
Databáze:	MEDLINE
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