Spatiotemporal tracking of small extracellular vesicle nanotopology in response to physicochemical stresses revealed by HS-AFM.

Autor: Sajidah ES; Division of Nano Life Science in the Graduate School of Frontier Science Initiative, Kanazawa University, Kanazawa, Ishikawa, Japan., Lim K; WPI-Nano Life Science Institute, Kanazawa University, Kanazawa, Ishikawa, Japan., Yamano T; WPI-Nano Life Science Institute, Kanazawa University, Kanazawa, Ishikawa, Japan.; Department of Immunology, Kanazawa University Graduate School of Medical Sciences, Kanazawa, Ishikawa, Japan., Nishide G; Division of Nano Life Science in the Graduate School of Frontier Science Initiative, Kanazawa University, Kanazawa, Ishikawa, Japan., Qiu Y; Division of Nano Life Science in the Graduate School of Frontier Science Initiative, Kanazawa University, Kanazawa, Ishikawa, Japan., Yoshida T; WPI-Nano Life Science Institute, Kanazawa University, Kanazawa, Ishikawa, Japan.; Department of Immunology, Kanazawa University Graduate School of Medical Sciences, Kanazawa, Ishikawa, Japan., Wang H; Cell-Bionomics Research Unit, Institute for Frontier Science Initiative (INFINITI), Kanazawa University, Kanazawa, Ishikawa, Japan., Kobayashi A; Cell-Bionomics Research Unit, Institute for Frontier Science Initiative (INFINITI), Kanazawa University, Kanazawa, Ishikawa, Japan., Hazawa M; WPI-Nano Life Science Institute, Kanazawa University, Kanazawa, Ishikawa, Japan.; Cell-Bionomics Research Unit, Institute for Frontier Science Initiative (INFINITI), Kanazawa University, Kanazawa, Ishikawa, Japan., Dewi FRP; Cell-Bionomics Research Unit, Institute for Frontier Science Initiative (INFINITI), Kanazawa University, Kanazawa, Ishikawa, Japan., Hanayama R; WPI-Nano Life Science Institute, Kanazawa University, Kanazawa, Ishikawa, Japan.; Department of Immunology, Kanazawa University Graduate School of Medical Sciences, Kanazawa, Ishikawa, Japan., Ando T; WPI-Nano Life Science Institute, Kanazawa University, Kanazawa, Ishikawa, Japan., Wong RW; Division of Nano Life Science in the Graduate School of Frontier Science Initiative, Kanazawa University, Kanazawa, Ishikawa, Japan.; WPI-Nano Life Science Institute, Kanazawa University, Kanazawa, Ishikawa, Japan.; Cell-Bionomics Research Unit, Institute for Frontier Science Initiative (INFINITI), Kanazawa University, Kanazawa, Ishikawa, Japan.
Jazyk: angličtina
Zdroj: Journal of extracellular vesicles [J Extracell Vesicles] 2022 Nov; Vol. 11 (11), pp. e12275.
DOI: 10.1002/jev2.12275
Abstrakt: Small extracellular vesicles (sEVs) play a crucial role in local and distant cell communication. The intrinsic properties of sEVs make them compatible biomaterials for drug delivery, vaccines, and theranostic nanoparticles. Although sEV proteomics have been robustly studied, a direct instantaneous assessment of sEV structure dynamics remains difficult. Here, we use the high-speed atomic force microscopy (HS-AFM) to evaluate nanotopological changes of sEVs with respect to different physicochemical stresses including thermal stress, pH, and osmotic stress. The sEV structure is severely altered at high-temperature, high-pH, or hypertonic conditions. Surprisingly, the spherical shape of the sEVs is maintained in acidic or hypotonic environments. Real-time observation by HS-AFM imaging reveals an irreversible structural change in the sEVs during transition of pH or osmolarity. HS-AFM imaging provides both qualitative and quantitative data at high spatiotemporal resolution (nanoscopic and millisecond levels). In summary, our study demonstrates the feasibility of HS-AFM for structural characterization and assessment of nanoparticles.
(© 2022 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje