UVC, UVB and UVA susceptibility of Phi6 and its suitability as a SARS-CoV-2 surrogate.

Autor:	Weyersberg L; Ulm University of Applied Sciences, Department of Medical Engineering and Mechatronics, Albert Einstein-Allee 55, D-89081 Ulm, Germany., Klemens E; Ulm University of Applied Sciences, Department of Medical Engineering and Mechatronics, Albert Einstein-Allee 55, D-89081 Ulm, Germany., Buehler J; Ulm University of Applied Sciences, Department of Medical Engineering and Mechatronics, Albert Einstein-Allee 55, D-89081 Ulm, Germany., Vatter P; Ulm University of Applied Sciences, Department of Medical Engineering and Mechatronics, Albert Einstein-Allee 55, D-89081 Ulm, Germany., Hessling M; Ulm University of Applied Sciences, Department of Medical Engineering and Mechatronics, Albert Einstein-Allee 55, D-89081 Ulm, Germany.
Jazyk:	angličtina
Zdroj:	AIMS microbiology [AIMS Microbiol] 2022 Jul 08; Vol. 8 (3), pp. 278-291. Date of Electronic Publication: 2022 Jul 08 (Print Publication: 2022).
DOI:	10.3934/microbiol.2022020
Abstrakt:	For SARS-CoV-2 disinfection systems or applications that are based on UVC, UVB or UVA irradiation, it would be desirable to have a SARS-CoV-2 surrogate for tests and development, which does not require a laboratory with a high biosafety level. The bacteriophage Phi 6, an enveloped RNA virus like coronaviruses, is an obvious candidate for such a surrogate. In this study, UVC, UVB and UVA log-reduction doses for Phi6 are determined by plaque assay. Log-reduction doses for SARS-CoV-2 are retrieved from a literature research. Because of a high variability of the published results, median log-reduction doses are determined for defined spectral ranges and compared to Phi6 data in the same intervals. The measured Phi6 log-reduction doses for UVC (254 nm), UVB (311 nm) and UVA (365 nm) are 31.7, 980 and 14 684 mJ/cm 2 , respectively. The determined median log-reduction doses for SARS-CoV-2 are much lower, only about 1.7 mJ/cm 2 within the spectral interval 251-270 nm. Therefore, Phi6 can be photoinactivated by all UV wavelengths but it is much less UV sensitive compared to SARS-CoV-2 in all UV spectral ranges. Thus, Phi6 is no convincing SARS-CoV-2 surrogate in UV applications. Competing Interests: Conflict of interest: The authors declare no conflict of interest. (© 2022 the Author(s), licensee AIMS Press.)
Databáze:	MEDLINE
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