| Autor: |
Ma X; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, P.R. China., Wang Y; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, P.R. China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu, Sichuan, P.R. China., Li X; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, P.R. China., Wang J; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, P.R. China., Wang B; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, P.R. China., Lin Y; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, P.R. China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu, Sichuan, P.R. China., Xiong Y; College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, P.R. China.; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu, Sichuan, P.R. China.; Key Laboratory of Sichuan Province for Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Exploitation, Chengdu, Sichuan, P.R. China. |
| Abstrakt: |
As a member of the PGAMs family, PGAM2 has been proved to catalyze the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. However, it is unclear whether PGAM2 has a role in regulating differentiation in goat intramuscular preadipocytes. Here, this study was carried to clone the open reading frame (ORF) of goat PGAM2 , elucidate its molecular and expressional characteristics, and evaluate the involvement in adipogenesis of intramuscular preadipocytes. According to our findings, the cloned goat PGAM2 gene was 784 bp in full length, including 762 bp of ORF and encoding a protein of 253 amino acids. The expressional level of PGAM2 peaked at 48 hours after induced adipogenic differentiation and was highest in the skeletal muscle of triceps. Moreover, overexpression of PGAM2 transfected by its overexpression plasmid promotes lipid accumulation of goat intramuscular adipocyte as shown by Oil Red O and bodipy staining, accompanied by up-regulating the mRNA levels of peroxisome proliferator-activated receptor γ ( PPARγ ) ( p < 0.001), sterol regulatory element-binding protein 1 ( SREBP1 ) ( p < 0.001), CCAAT/Enhancer-binding protein α ( C/EBPα ) ( p < 0.01) and lipoprotein lipase ( LPL ) ( p < 0.01). Taken together, these findings indicate that PGAM2 is a positive regulator for goat intramuscular adipocytes and provide new insights into improvement intramuscular fat deposition in goat meat. |
