Intertubular morphometric and ultrastructural testes analyses in mdx mice.

Autor:	Braz JKFDS; Escola Multicampi de Ciências Médicas do RN, Universidade Federal do Rio Grande do Norte, Caicó, RN, Brasil., Gomes VA; Departamento de Ciências da Saúde, Universidade Federal de Campina Grande, Campina Grande, PB, Brasil., Siman VA; Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, MG, Brasil., da Matta SLP; Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, MG, Brasil., Clebis NK; Departamento de Morfologia, Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil., de Oliveira MF; Departamento de Ciência Animal, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brasil., Assis AC; Departamento de Cirurgia, Faculdade de Medicina Veterinária e Ciência Animal, Universidade de São Paulo, São Paulo, SP, Brasil., Morais DB; Departamento de Morfologia, Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil., de Moura CEB; Departamento de Ciência Animal, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brasil.
Jazyk:	angličtina
Zdroj:	Animal reproduction [Anim Reprod] 2022 Oct 24; Vol. 19 (3), pp. e20210124. Date of Electronic Publication: 2022 Oct 24 (Print Publication: 2022).
DOI:	10.1590/1984-3143-AR2021-0124
Abstrakt:	Duchenne Muscular Dystrophy (DMD) reproductive alterations and the influence of antioxidant treatments may aid in understanding morphometry testicular quantification. In this context, the aim of the present study was to characterize the intertubular compartment (ITC) morphometry of animal testes in mdx mice supplemented with ascorbic acid (AA). Sixteen mice were used, namely the C57BL/10 (non-dystrophic) and C57BL/10Mdx (dystrophic) lineages, distributed into the following groups: Control (C60), Dystrophic (D60), Control supplemented with AA (CS60), Dystrophic supplemented with AA (DS60). A total of 200 mg/kg of AA were administered to mice for 30 days. Subsequently, the testicles were collected, weighed, and fragmented. The obtained fragments were fixed in Karnovsky's solution (pH 7.2) and embedded in historesin for morphometric and transmission electron microscopy assessments. Leydig cells were hypertrophic in the D60 group, but was reverted by AA supplementation in the DS60 group. The DS60 group also exhibited increased intertubular volume compared to the CS60 group. The ultrastructural images identified multilamellar bodies in dystrophic animals (lipid storage) and telocyte cells (transport substances) in both control and dystrophic animals. Morphometric alterations were, therefore, noted in the intertubular compartment due to Duchenne muscular dystrophy (DMD), with AA administration capable of altering Leydig cells in this condition. Competing Interests: Conflicts of interest: The authors have no conflict of interest to declare.
Databáze:	MEDLINE
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