Phenotypic and genotypic investigation of metallo-β-lactamases in Pseudomonas aeruginosa clinical isolates in Bushehr, Iran.

Autor: Seyedi M; Department of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.; Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran., Yousefi F; Department of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.; The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran., Naeimi B; Department of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.; The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran., Tajbakhsh S; Department of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.; The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran.
Jazyk: angličtina
Zdroj: Iranian journal of basic medical sciences [Iran J Basic Med Sci] 2022 Oct; Vol. 25 (10), pp. 1196-1200.
DOI: 10.22038/IJBMS.2022.64359.14154
Abstrakt: Objectives: Production of metallo-β-lactamases (MBLs) is an important mechanism of resistance to carbapenems. This study aimed to detect the MBL-producing Pseudomonas aeruginosa clinical isolates and to investigate the presence of bla VIM , bla IMP , bla SPM , bla NDM , bla GIM , bla AIM , and bla SIM genes in these isolates in Bushehr, Iran.
Materials and Methods: A total of 169 P. aeruginosa clinical isolates were collected from three hospitals in Bushehr. The modified carbapenem inactivation method (mCIM) was used for the phenotypic detection of carbapenemase production. A combination disk test (CDT) was performed for the phenotypic detection of MBL production. To investigate the presence of bla VIM , bla IMP , bla SPM , bla NDM , bla GIM , bla AIM , and bla SIM genes, PCR and sequencing was carried out.
Results: Based on the results of mCIM, 40 (23.7%) of 169 isolates were carbapenemase producers. CDT revealed that 26 (15.4%) isolates were MBL producers. bla IMP , bla NDM , and bla VIM genes were found in 18 (69.2%), 8 (30.8%), and 1 (3.8%) of the MBL-producing isolates, respectively. Coexistence of bla IMP and bla NDM was observed in 2 (7.7%) MBL-producing isolates. Among all 169 P. aeruginosa isolates, 23 (13.6%) harbored bla NDM , 18 (10.6%) carried bla IMP , and 1 (0.6%) carried the bla VIM gene. bla SPM , bla GIM , bla AIM , and bla SIM were not found in the present study.
Conclusion: bla NDM , bla IMP , and bla VIM genes were detected in this study, which could be a warning sign about the prevalence of these genes among P. aeruginosa clinical isolates in our region. Proper monitoring and detection of MBL-producing isolates are essential steps to prevent the spread of these isolates.
Competing Interests: The authors declare that there are no conflicts of interest.
Databáze: MEDLINE
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