Comparative analysis of within-host diversity among vaccinated COVID-19 patients infected with different SARS-CoV-2 variants.

Autor:	Al-Khatib HA; Biomedical Research Center, Qatar University, Doha 2713, Qatar., Smatti MK; Biomedical Research Center, Qatar University, Doha 2713, Qatar., Ali FH; Biomedical Research Center, Qatar University, Doha 2713, Qatar., Zedan HT; Biomedical Research Center, Qatar University, Doha 2713, Qatar., Thomas S; Biomedical Research Center, Qatar University, Doha 2713, Qatar., Ahmed MN; Biomedical Research Center, Qatar University, Doha 2713, Qatar., El-Kahlout RA; Virology Laboratory, Hamad Medical Corporation, Doha 3050, Qatar., Al Bader MA; National Reference Laboratory, Ministry of Public Health, Doha 42, Qatar., Elgakhlab D; Qatar Biobank, Qatar Foundation, Doha, Qatar., Coyle PV; Virology Laboratory, Hamad Medical Corporation, Doha 3050, Qatar., Abu-Raddad LJ; Weill Cornell Medicine-Qatar, Qatar Foundation, Doha, Qatar., Al Thani AA; Biomedical Research Center, Qatar University, Doha 2713, Qatar.; Department of Biomedical Sciences, College of Health Sciences, Qatar University, Doha, Qatar., Yassine HM; Biomedical Research Center, Qatar University, Doha 2713, Qatar.; Department of Biomedical Sciences, College of Health Sciences, Qatar University, Doha, Qatar.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2022 Nov 18; Vol. 25 (11), pp. 105438. Date of Electronic Publication: 2022 Oct 25.
DOI:	10.1016/j.isci.2022.105438
Abstrakt:	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a rapidly evolving RNA virus that mutates within hosts and exists as viral quasispecies. Here, we evaluated the within-host diversity among vaccinated and unvaccinated individuals (n = 379) infected with different SARS-CoV-2 Variants of Concern. The majority of samples harbored less than 14 intra-host single-nucleotide variants (iSNVs). A deep analysis revealed a significantly higher intra-host diversity in Omicron samples than in other variants (p value < 0.05). Vaccination status and type had a limited impact on intra-host diversity except for Beta-B.1.315 and Delta-B.1.617.2 vaccinees, who exhibited higher diversity than unvaccinated individuals (p values: <0.0001 and <0.0021, respectively). Three immune-escape mutations were identified: S255F in Delta and R346K and T376A in Omicron-B.1.1.529. The latter 2 mutations were fixed in BA.1 and BA.2 genomes, respectively. Overall, the relatively higher intra-host diversity among vaccinated individuals and the detection of immune-escape mutations, despite being rare, suggest a potential vaccine-induced immune pressure in vaccinated individuals. Competing Interests: The authors declare no competing interests. (© 2022 The Author(s).)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu