Evaluation of MMR live attenuated vaccine oncolytic potential using Ehrlich ascites carcinoma in a murine model.

Autor: Hassan ST; Laboratory Evaluation Administration, Egyptian Drug Authority, Giza, 12654, Egypt. sara.t.hassan@std.pharma.cu.edu.eg., Mohamed AF; International Center for Training and Advanced Researches (ICTAR-Egypt), Cairo, Egypt., AbdelAllah NH; Laboratory Evaluation Administration, Egyptian Drug Authority, Giza, 12654, Egypt., Zedan H; Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt.
Jazyk: angličtina
Zdroj: Medical oncology (Northwood, London, England) [Med Oncol] 2022 Oct 29; Vol. 40 (1), pp. 6. Date of Electronic Publication: 2022 Oct 29.
DOI: 10.1007/s12032-022-01866-x
Abstrakt: MMR vaccine is a common vaccine that contains oncolytic viruses (Measles, Mumps, and Rubella) and could be used as a potential anti-cancer treatment. In this study, we assessed the anti-tumor activity of the MMR vaccine against Ehrlich ascites carcinoma (EAC) solid tumor induced in mice. The in vitro assay showed that vaccine IC50 in EAC was approximately 200 CCID 50. The vaccine was intratumorally administrated twice weekly in EAC-bearing mice. The antitumor response of the vaccine was measured by tumor growth, survival rate, histopathologic examination, flow cytometry analysis, and body biochemical parameters. The MMR vaccine demonstrated a substantial reduction of tumor growth and prolongation of life span as well. The proliferation marker was significantly lower in the vaccine-treated group. Moreover, the apoptosis key parameter Casp-3 was also higher in the vaccine-treated group. The vaccine somewhat restored the deterioration of the biochemical parameters (LDH, GOT, GPT, MDA, NO, and PON-1) in the tumor-bearing mice. Finally, this study indicated the potential antitumor effect of MMR vaccine via anti‑proliferative, apoptotic activities, and modulating the antioxidant parameters. This study opens a new field of inquiry for future research on the vaccine's anti-cancer properties.
(© 2022. The Author(s).)
Databáze: MEDLINE
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