Assessment of Cell Cycle and Induction of Apoptosis by Shiga-like Toxin Produced by Escherichia coli O157:H7 in T47D Breast Cancer Cells Using Flow Cytometry.
| Autor: | Suardana IW; Department of Preventive Veterinary Medicine, Faculty of Veterinary Medicine, Udayana University. Jl. P.B. Sudirman, Denpasar-Bali. 80234. Indonesia., Swacita IBN; Department of Preventive Veterinary Medicine, Faculty of Veterinary Medicine, Udayana University. Jl. P.B. Sudirman, Denpasar-Bali. 80234. Indonesia., Pinatih KJP; Department of Clinical Microbiology, Faculty of Medicine, Udayana University. Jl. P.B.Sudirman, Denpasar-Bali. 80234. Indonesia., Suharsono H; Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Udayana University. Jl. PB. Sudirman, Denpasar-Bali. 80234. Indonesia., Widiasih DA; Department of Veterinary Public Health, Faculty of Veterinary Medicine, Gadjah Mada University. Jl. Fauna No. 2 Karangmalang, Yogyakarta 55281 Indonesia. |
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| Jazyk: | angličtina |
| Zdroj: | Asian Pacific journal of cancer prevention : APJCP [Asian Pac J Cancer Prev] 2022 Oct 01; Vol. 23 (10), pp. 3247-3252. Date of Electronic Publication: 2022 Oct 01. |
| DOI: | 10.31557/APJCP.2022.23.10.3247 |
| Abstrakt: | Background: The low general toxicity against tumors expressing globotriaosylceramide (Gb3) and Shiga-like toxins produced by E. coli have been proposed as an anti-cancer therapy because of their specific target. This study aimed to determine the potency of the local strains of E. coli O157:H7 isolated from humans and cattle as a new breast cancer therapy by analyzing the cell cycle's inhibition and apoptosis induction. Material and Methods: Approximately 10 cultured T47D cells were subjected to Shiga-like toxin produced by four local isolates of E. coli O157:H7, including KL-48 (2) from humans, and SM-25 (1), SM-7 (1), DS-21 (4) from cattle. Using ATCC 43894 as a control, the treatment was observed for 24 h by two replications. In addition, a FITC-Annexin V and PI assay were used to observe apoptosis and necrosis effect, as well as to analyze the cell cycle using propidium iodide (PI) staining. Results: The results showed the toxicity effect of Shiga in the human T47 D cells line. The viability of the cells is subjected to Shiga-like toxins produced by KL-48 (2), SM7 (1), ATCC 43894, SM-25 (1), and DS-21 (4) isolates decreased with 15.20, 16.36, 22.17, 22.64, and 33.86%, in contrary to control of 94.36%. These were supported by the cells entering the late apoptosis of the cell cycle through each isolate with 67.66, 62.60, 63.68, 63.90, and 54.74%, and a control of 0.01%. Also, the necrosis cell for each treatment of 12.73, 19.3, 10.84, 10.53, and 4.86% was higher than the control of 5.51%. These were confirmed by the higher percentage of the cells treated with toxins of KL-48 (2), SM7(1), ATCC 43894, SM-25 (1), and DS-21 (4), which entered G0-G1 of the cell cycle phase with 66.41, 63.37, 61.52, 55.36, and 47.28%, respectively, than control of 40.69%. Additionally, the toxicity effect was supported by an increase in the cells entering the S and the G2-M phase of the cycle for each treatment. Conclusion: It is concluded that the Shiga-like toxin produced by E. coli O157:H7 local isolates can be developed as a drug against breast cancer based on its effect to arrest induction of the cell cycle and inducing apoptosis. |
| Databáze: | MEDLINE |
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