A cryogenic, coincident fluorescence, electron, and ion beam microscope.

Autor:	Boltje DB; Department of Imaging Physic, Delft University of Technology, Delft, Netherlands.; Delmic B.V, Delft, Netherlands., Hoogenboom JP; Department of Imaging Physic, Delft University of Technology, Delft, Netherlands., Jakobi AJ; Kavli Institute of Nanoscience, Delft University of Technology, Delft, Netherlands., Jensen GJ; California Institute of Technology, Pasadena, United States.; Brigham Young University, Provo, United States., Jonker CTH; Delmic B.V, Delft, Netherlands., Kaag MJ; Department of Imaging Physic, Delft University of Technology, Delft, Netherlands., Koster AJ; Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, Netherlands., Last MGF; Delmic B.V, Delft, Netherlands.; Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, Netherlands., de Agrela Pinto C; Kavli Institute of Nanoscience, Delft University of Technology, Delft, Netherlands., Plitzko JM; CryoEM Technology, Max Planck Institute of Biochemistry, Martinsried, Germany., Raunser S; Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund, Germany., Tacke S; Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund, Germany., Wang Z; Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund, Germany., van der Wee EB; Department of Imaging Physic, Delft University of Technology, Delft, Netherlands., Wepf R; Centre for Microscopy and Microanalysis, University of Queensland, Brisbane, Australia., den Hoedt S; Delmic B.V, Delft, Netherlands.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2022 Oct 28; Vol. 11. Date of Electronic Publication: 2022 Oct 28.
DOI:	10.7554/eLife.82891
Abstrakt:	Cryogenic electron tomography (cryo-ET) combined with subtomogram averaging, allows in situ visualization and structure determination of macromolecular complexes at subnanometre resolution. Cryogenic focused ion beam (cryo-FIB) micromachining is used to prepare a thin lamella-shaped sample out of a frozen-hydrated cell for cryo-ET imaging, but standard cryo-FIB fabrication is blind to the precise location of the structure or proteins of interest. Fluorescence-guided focused ion beam (FIB) milling at target locations requires multiple sample transfers prone to contamination, and relocation and registration accuracy is often insufficient for 3D targeting. Here, we present in situ fluorescence microscopy-guided FIB fabrication of a frozen-hydrated lamella to address this problem: we built a coincident three-beam cryogenic correlative microscope by retrofitting a compact cryogenic microcooler, custom positioning stage, and an inverted widefield fluorescence microscope (FM) on an existing FIB scanning electron microscope. We show FM controlled targeting at every milling step in the lamella fabrication process, validated with transmission electron microscope tomogram reconstructions of the target regions. The ability to check the lamella during and after the milling process results in a higher success rate in the fabrication process and will increase the throughput of fabrication for lamellae suitable for high-resolution imaging. Competing Interests: DB is an employee of Delmic B.V, JH, Sd has a financial interest in Delmic B.V, AJ, GJ, MK, AK, Cd, JP, SR, ST, ZW, Ev, RW No competing interests declared, CJ, ML were employees of Delmic B.V (© 2022, Boltje et al.)
Databáze:	MEDLINE
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