Remodeling of the cell wall as a drought-tolerance mechanism of a soybean genotype revealed by global gene expression analysis.

Autor: Coutinho FS; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais Brazil., Rodrigues JM; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais Brazil., Lima LL; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais Brazil., Mesquita RO; Departamento de Fitotecnia, Universidade Federal do Ceará, Fortaleza, Ceará Brazil., Carpinetti PA; Núcleo de Análise de Biomoléculas (NuBioMol), Universidade Federal de Viçosa, Viçosa, Minas Gerais Brazil., Machado JPB; Instituto de Ciências Agrárias, Universidade Federal de Viçosa, Florestal, Minas Gerais Brazil., Vital CE; Núcleo de Análise de Biomoléculas (NuBioMol), Universidade Federal de Viçosa, Viçosa, Minas Gerais Brazil., Vidigal PM; Núcleo de Análise de Biomoléculas (NuBioMol), Universidade Federal de Viçosa, Viçosa, Minas Gerais Brazil., Ramos MES; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais Brazil., Maximiano MR; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF Brazil.; Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais Brazil., Mehta A; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF Brazil., Oliveira MGA; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais Brazil., Fontes EPB; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais Brazil., de Oliveira Ramos HJ; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais Brazil.
Jazyk: angličtina
Zdroj: ABIOTECH [aBIOTECH] 2021 Apr 05; Vol. 2 (1), pp. 14-31. Date of Electronic Publication: 2021 Apr 05 (Print Publication: 2021).
DOI: 10.1007/s42994-021-00043-4
Abstrakt: Drought stress is major abiotic stress that affects soybean production. Therefore, it is widely desirable that soybean becomes more tolerant to stress. To provide insights into regulatory mechanisms of the stress response, we compared the global gene expression profiles from leaves of two soybean genotypes that display different responses to water-deficit (BR 16 and Embrapa 48, drought-sensitive and drought-tolerant, respectively). After the RNA-seq analysis, a total of 5335 down-regulated and 3170 up-regulated genes were identified in the BR16. On the other hand, the number of genes differentially expressed was markedly lower in the Embrapa 48, 355 up-regulated and 471 down-regulated genes. However, induction and expression of protein kinases and transcription factors indicated signaling cascades involved in the drought tolerance. Overall, the results suggest that the metabolism of pectin is differently modulated in response to drought stress and may play a role in the soybean defense mechanism against drought. This occurs via an increase of the cell wall plasticity and crosslink, which contributed to a higher hydraulic conductance ( K f ) and relative water content (RWC%). The drought-tolerance mechanism of the Embrapa 48 genotype involves remodeling of the cell wall and increase of the hydraulic conductance to the maintenance of cell turgor and metabolic processes, resulting in the highest leaf RWC, photosynthetic rate ( A ), transpiration ( E ) and carboxylation ( A / C i ). Thus, we concluded that the cell wall adjustment under drought is important for a more efficient water use which promoted a more active photosynthetic metabolism, maintaining higher plant growth under drought stress.
Supplementary Information: The online version contains supplementary material available at 10.1007/s42994-021-00043-4.
Competing Interests: Conflict of interestOn behalf of all authors, the corresponding author states that there is no conflict of interest.
(© Agricultural Information Institute, Chinese Academy of Agricultural Sciences 2021.)
Databáze: MEDLINE
Externí odkaz: