Genomic Characterization of Lactobacillus delbrueckii Strains with Probiotics Properties.

Autor:	De Jesus LCL; Department of Genetics, Ecology and Evolution, Federal University of Minas Gerais, Belo Horizonte, Brazil., Aburjaile FF; Department of Preventive Veterinary Medicine, Federal University of Minas Gerais, Belo Horizonte, Brazil., Sousa TJ; Department of Genetics, Ecology and Evolution, Federal University of Minas Gerais, Belo Horizonte, Brazil., Felice AG; Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, Brazil., Soares SC; Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, Brazil., Alcantara LCJ; Department of Genetics, Ecology and Evolution, Federal University of Minas Gerais, Belo Horizonte, Brazil.; Flavivirus Laboratory, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil., Azevedo VAC; Department of Genetics, Ecology and Evolution, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in bioinformatics [Front Bioinform] 2022 Jun 06; Vol. 2, pp. 912795. Date of Electronic Publication: 2022 Jun 06 (Print Publication: 2022).
DOI:	10.3389/fbinf.2022.912795
Abstrakt:	Probiotics are health-beneficial microorganisms with mainly immunomodulatory and anti-inflammatory properties. Lactobacillus delbrueckii species is a common bacteria used in the dairy industry, and their benefits to hosting health have been reported. This study analyzed the core genome of nine strains of L. delbrueckii species with documented probiotic properties, focusing on genes related to their host health benefits. For this, a combined methodology including several software and databases (BPGA, SPAAN, BAGEL4, BioCyc, KEEG, and InterSPPI) was used to predict the most important characteristics related to L. delbrueckii strains probiose. Comparative genomics analyses revealed that L. delbrueckii probiotic strains shared essential genes related to acid and bile stress response and antimicrobial activity. Other standard features shared by these strains are surface layer proteins and extracellular proteins-encoding genes, with high adhesion profiles that interacted with human proteins of the inflammatory signaling pathways (TLR2/4-MAPK, TLR2/4-NF-κB, and NOD-like receptors). Among these, the PrtB serine protease appears to be a strong candidate responsible for the anti-inflammatory properties reported for these strains. Furthermore, genes with high proteolytic and metabolic activity able to produce beneficial metabolites, such as acetate, bioactive peptides, and B-complex vitamins were also identified. These findings suggest that these proteins can be essential in biological mechanisms related to probiotics' beneficial effects of these strains in the host. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 De Jesus, Aburjaile, Sousa, Felice, Soares, Alcantara and Azevedo.)
Databáze:	MEDLINE
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