Association of microRNAs With Embryo Development and Fertilization in Women Undergoing Subfertility Treatments: A Pilot Study.

Autor:	Butler AE; Research Department, Royal College of Surgeons Ireland, Adliya, Bahrain., Cunningham TK; Academic Diabetes, Endocrinology and Metabolism, Hull York Medical School, University of Hull, Hull, United Kingdom.; The Hull IVF Unit. Women's and Children's Hospital, Hull Royal Infirmary, Hull, United Kingdom., Ramachandran V; Division of Research, MicroRNA Core Laboratory, Weill Cornell Medicine-Qatar, Qatar Foundation, Education City, Doha, Qatar., Diboun I; Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), Doha, Qatar., Halama A; Division of Research, Weill Cornell Medicine-Qatar, Qatar Foundation, Education City, Doha, Qatar., Sathyapalan T; Academic Diabetes, Endocrinology and Metabolism, Hull York Medical School, University of Hull, Hull, United Kingdom., Najafi-Shoushtari SH; Division of Research, MicroRNA Core Laboratory, Weill Cornell Medicine-Qatar, Qatar Foundation, Education City, Doha, Qatar.; Department of Cell and Developmental Biology, Weill Cornell Medicine, New York, NY, United States., Atkin SL; Research Department, Royal College of Surgeons Ireland, Adliya, Bahrain.
Jazyk:	angličtina
Zdroj:	Frontiers in reproductive health [Front Reprod Health] 2021 Sep 23; Vol. 3, pp. 719326. Date of Electronic Publication: 2021 Sep 23 (Print Publication: 2021).
DOI:	10.3389/frph.2021.719326
Abstrakt:	Objective: Small non-coding RNAs, known as microRNAs (miRNAs), have emerging regulatory functions within the ovary that have been related to fertility. This study was undertaken to determine if circulating miRNAs reflect the changes associated with the parameters of embryo development and fertilization. Methods: In this cross-sectional pilot study. Plasma miRNAs were collected from 48 sequentially presenting women in the follicular phase prior to commencing in vitro fertilization (IVF). Circulating miRNAs were measured using locked nucleic acid (LNA)-based quantitative PCR (qPCR), while an updated miRNA data set was used to determine their level of expression. Results: Body mass index and weight were associated with the miRNAs let7b-3p and miR-375, respectively ( p < 0.05), with the same relationship being found between endometrium thickness at oocyte retrieval and miR-885-5p and miR-34a-5p ( p < 0.05). In contrast, miR-1260a was found to be inversely associated with anti-Mullerian hormone (AMH; p = 0.007), while miR-365a-3p, miR122-5p, and miR-34a-5p correlated with embryo fertilization rates ( p < 0.05). However, when omitting cases of male infertility ( n = 15), miR122-5p remained significant ( p < 0.05), while miR-365a-3p and miR-34a-5p no longer differed; interestingly, however, miR1260a and mir93.3p became significant ( p = 0.0087/0.02, respectively). Furthermore, age was negatively associated with miR-335-3p, miR-28-5p, miR-155-5p, miR-501-3p, and miR-497-5p ( p < 0.05). Live birth rate was negatively associated with miR-335-3p, miR-100-5p, miR-497-5p, let-7d, and miR-574-3p ( p < 0.05), but these were not significant when age was accounted for.However, with the exclusion of male factor infertility, all those miRNAs were no longer significant, though miR.150.5p emerged as significant ( p = 0.042). A beta-regression model identified miR-1260a, miR-486-5p, and miR-132-3p ( p < 0.03, p = 0.0003, p < 0.00001, respectively) as the most predictive for fertilization rate. Notably, changes in detectable miRNAs were not linked to cleavage rate, top quality embryos (G3D3), and blastocyst or antral follicle count. An ingenuity pathway analysis showed that miRNAs associated with age were also associated with the variables found in reproductive system diseases. Conclusion: Plasma miRNAs prior to the IVF cycle were associated with differing demographic and IVF parameters, including age, and may be predictive biomarkers of fertilization rate. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Butler, Cunningham, Ramachandran, Diboun, Halama, Sathyapalan, Najafi-Shoushtari and Atkin.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu