Effect of fixed orthodontic appliances on gingival status and oral microbiota: a pilot study.

Autor: Marincak Vrankova Z; Clinic of Stomatology, Institution Shared with St. Anne's University Hospital, Faculty of Medicine, Masaryk University, Pekařská 53, 656 91, Brno, Czech Republic.; Clinic of Maxillofacial Surgery, Institution Shared with University Hospital Brno, Faculty of Medicine, Jihlavska 20, 625 00, Brno, Czech Republic.; RECETOX, Faculty of Science, Masaryk University, Kotlarska 2, Brno, Czech Republic., Rousi M; Clinic of Stomatology, Institution Shared with St. Anne's University Hospital, Faculty of Medicine, Masaryk University, Pekařská 53, 656 91, Brno, Czech Republic.; Margarita Rousi Orthodontic Center, Athinon 91, 2035, Nicosia, Cyprus., Cvanova M; RECETOX, Faculty of Science, Masaryk University, Kotlarska 2, Brno, Czech Republic.; Institute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic., Gachova D; RECETOX, Faculty of Science, Masaryk University, Kotlarska 2, Brno, Czech Republic., Ruzicka F; Department of Microbiology, Institution Shared with St. Anne's University Hospital and Faculty of Medicine, Masaryk University, Pekařská 53, 656 91, Brno, Czech Republic., Hola V; Department of Microbiology, Institution Shared with St. Anne's University Hospital and Faculty of Medicine, Masaryk University, Pekařská 53, 656 91, Brno, Czech Republic., Lochman J; Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic., Izakovicova Holla L; Clinic of Stomatology, Institution Shared with St. Anne's University Hospital, Faculty of Medicine, Masaryk University, Pekařská 53, 656 91, Brno, Czech Republic., Brysova A; Clinic of Stomatology, Institution Shared with St. Anne's University Hospital, Faculty of Medicine, Masaryk University, Pekařská 53, 656 91, Brno, Czech Republic., Borilova Linhartova P; Clinic of Stomatology, Institution Shared with St. Anne's University Hospital, Faculty of Medicine, Masaryk University, Pekařská 53, 656 91, Brno, Czech Republic. petra.linhartova@recetox.muni.cz.; Clinic of Maxillofacial Surgery, Institution Shared with University Hospital Brno, Faculty of Medicine, Jihlavska 20, 625 00, Brno, Czech Republic. petra.linhartova@recetox.muni.cz.; RECETOX, Faculty of Science, Masaryk University, Kotlarska 2, Brno, Czech Republic. petra.linhartova@recetox.muni.cz.
Jazyk: angličtina
Zdroj: BMC oral health [BMC Oral Health] 2022 Oct 27; Vol. 22 (1), pp. 455. Date of Electronic Publication: 2022 Oct 27.
DOI: 10.1186/s12903-022-02511-9
Abstrakt: Background: This pilot study aimed to investigate how fixed orthodontic appliances simultaneously applied on the upper and lower arches affect the oral environment in the medium term.
Methods: The oral status of 30 orthodontic patients was evaluated using the number of decay-missing-filled teeth (DMFT), plaque (PI), and gingival indices (GI) before bonding of fixed orthodontic appliances (T0) and during the therapy (T1). Besides, the gingival crevicular fluid (GCF) and a dental plaque were collected. Samples were analyzed for selected Candida sp. and for 10 selected oral bacteria using mass spectroscopy and multiplex polymerase chain reaction, respectively.
Results: In 60% of patients, deterioration of the oral status (demonstrated by the increase in PI) was recorded (p < 0.05). Moreover, the changes in PI correlated with those of GI (p < 0.001). At the T1 time point, the mean representation of Actinomyces sp. in the total prokaryotic DNA in GCF and dental plaque of individual patients increased compared to T0 (p < 0.05). The probability of finding any of the 7 selected periodontal bacteria combined with Candida sp. was 10 times higher in patients in whom PI deteriorated between T0 and T1 (p < 0.01).
Conclusions: Changes in the oral microbial diversity and an increase in PI were observed in the medium term after bonding of orthodontic appliance. Our study highlights the importance of a complex approach in this type of research as the association between clinical characteristics and combined microbial parameters is higher than when evaluated separately.
(© 2022. The Author(s).)
Databáze: MEDLINE
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