Three-dimensional characterization of developing and adult ocular vasculature in mice using in toto clearing.

Autor: Darche M; Clinical Investigation Center 1423, Quinze-Vingts hospital, INSERM-DHOS, 28 rue de Charenton, Paris, F-75012, France.; Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012, Paris, France., Verschueren A; Clinical Investigation Center 1423, Quinze-Vingts hospital, INSERM-DHOS, 28 rue de Charenton, Paris, F-75012, France.; Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012, Paris, France., Belle M; Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012, Paris, France., Boucherit L; Clinical Investigation Center 1423, Quinze-Vingts hospital, INSERM-DHOS, 28 rue de Charenton, Paris, F-75012, France.; Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012, Paris, France., Fouquet S; Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012, Paris, France., Sahel JA; Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012, Paris, France.; Department of Ophthalmology, Fondation Ophtalmologique Adolphe De Rothschild, F-75019, Paris, France.; Department of Ophthalmology, The University of Pittsburgh School of Medicine, Pittsburgh, PA, 15213, USA., Chédotal A; Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012, Paris, France., Cascone I; Univ Paris Est Créteil, INSERM, IMRB, F-94010, Créteil, France.; AP-HP, Groupe hospitalo-universitaire Chenevier Mondor, Centre d'investigation clinique Biothérapie, F-94010, Créteil, France., Paques M; Clinical Investigation Center 1423, Quinze-Vingts hospital, INSERM-DHOS, 28 rue de Charenton, Paris, F-75012, France. mpaques@15-20.fr.; Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012, Paris, France. mpaques@15-20.fr.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2022 Oct 27; Vol. 5 (1), pp. 1135. Date of Electronic Publication: 2022 Oct 27.
DOI: 10.1038/s42003-022-04104-2
Abstrakt: The ocular vasculature is critically involved in many blinding diseases and is also a popular research model for the exploration of developmental and pathological angiogenesis. The development of ocular vessels is a complex, finely orchestrated sequence of events, involving spatial and temporal coordination of hyaloid, choroidal and retinal networks. Comprehensive studies of the tridimensional dynamics of microvascular remodeling are limited by the fact that preserving the spatial disposition of ocular vascular networks is cumbersome using classical histological procedures. Here, we demonstrate that light-sheet fluorescence microscopy (LFSM) of cleared mouse eyes followed by extensive virtual dissection offers a solution to this problem. To the best of our knowledge, this is the first 3D quantification of the evolution of the hyaloid vasculature and of post-occlusive venous remodeling together with the characterization of spatial distribution of various cell populations in ocular compartments, including the vitreous. These techniques will prove interesting to obtain other insights in scientific questions addressing organ-wide cell interactions.
(© 2022. The Author(s).)
Databáze: MEDLINE
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