Gauging the skin resident Leishmania parasites through a loop mediated isothermal amplification (LAMP) assay in post-kala-azar dermal leishmaniasis.

Autor: Ghosh P; Nutrition and Clinical Services Division, icddr,b, Dhaka, 1212, Bangladesh., Chowdhury R; Nutrition and Clinical Services Division, icddr,b, Dhaka, 1212, Bangladesh., Maruf S; Nutrition and Clinical Services Division, icddr,b, Dhaka, 1212, Bangladesh., Picado A; Foundation for Innovative New Diagnostics (FIND), Geneva, Switzerland., Hossain F; Nutrition and Clinical Services Division, icddr,b, Dhaka, 1212, Bangladesh., Owen SI; Department of Tropical Disease Biology, Liverpool School of Tropical Medicine (LSTM), Liverpool, UK., Nath R; Nutrition and Clinical Services Division, icddr,b, Dhaka, 1212, Bangladesh., Baker J; Nutrition and Clinical Services Division, icddr,b, Dhaka, 1212, Bangladesh., Hasnain MG; School of Medicine and Public Health, The University of Newcastle, Callaghan, NSW, Australia., Shomik MS; Nutrition and Clinical Services Division, icddr,b, Dhaka, 1212, Bangladesh., Ghosh D; Nutrition and Clinical Services Division, icddr,b, Dhaka, 1212, Bangladesh., Rashid M; National Heart Foundation and Research Institute, Mirpur, 1216, Bangladesh., Rashid MU; Nutrition and Clinical Services Division, icddr,b, Dhaka, 1212, Bangladesh., Sagar SK; Nutrition and Clinical Services Division, icddr,b, Dhaka, 1212, Bangladesh., Rahat MA; Nutrition and Clinical Services Division, icddr,b, Dhaka, 1212, Bangladesh., Basher A; Infectious Disease Hospital, Mohakhali, Dhaka, 1212, Bangladesh., Nath P; Infectious and Tropical Medicine Department, Mymensingh Medical College and Hospital (MMCH), Mymensingh, 2200, Bangladesh., Edwards T; Department of Tropical Disease Biology, Liverpool School of Tropical Medicine (LSTM), Liverpool, UK., Andrews JR; Stanford University School of Medicine, Stanford, CA, 94305, USA., Duthie MS; HDT Bio, Suite 280, 1616 Eastlake Ave E, Seattle, WA, 98102, USA., de Souza DK; Foundation for Innovative New Diagnostics (FIND), Geneva, Switzerland.; Department of Parasitology, College of Health Sciences, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana., Adams ER; Department of Tropical Disease Biology, Liverpool School of Tropical Medicine (LSTM), Liverpool, UK., Ndungu J; Foundation for Innovative New Diagnostics (FIND), Geneva, Switzerland., Cruz I; Foundation for Innovative New Diagnostics (FIND), Geneva, Switzerland.; National School of Public Health, CIBERINFEC, Instituto de Salud Carlos III ES, Madrid, Spain., Mondal D; Nutrition and Clinical Services Division, icddr,b, Dhaka, 1212, Bangladesh. din63d@icddrb.org.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Oct 27; Vol. 12 (1), pp. 18069. Date of Electronic Publication: 2022 Oct 27.
DOI: 10.1038/s41598-022-21497-6
Abstrakt: Despite the availability of highly sensitive polymerase chain reaction (PCR)-based methods, the dearth of remotely deployable diagnostic tools circumvents the early and accurate detection of individuals with post-kala-azar dermal leishmaniasis (PKDL). Here, we evaluate a design-locked loop-mediated isothermal amplification (LAMP) assay to diagnose PKDL. A total of 76 snip-skin samples collected from individuals with probable PKDL (clinical presentation and a positive rK39 rapid diagnostic test (RDT)) were assessed by microscopy, qPCR, and LAMP. An equal number of age and sex-matched healthy controls were included to determine the specificity of the LAMP assay. The LAMP assay with a Qiagen DNA extraction (Q-LAMP) showed a promising sensitivity of 72.37% (95% CI: 60.91-82.01%) for identifying the PKDL cases. LAMP assay sensitivity declined when the DNA was extracted using a boil-spin method. Q-qPCR showed 68.42% (56.75-78.61%) sensitivity, comparable to LAMP and with an excellent agreement, whereas the microscopy exhibited a weak sensitivity of 39.47% (28.44-51.35%). When microscopy and/or qPCR were considered the gold standard, Q-LAMP exhibited an elevated sensitivity of 89.7% (95% CI: 78.83-96.11%) for detection of PKDL cases and Bayesian latent class modeling substantiated the excellent sensitivity of the assay. All healthy controls were found to be negative. Notwithstanding the optimum efficiency of the LAMP assay towards the detection of PKDL cases, further optimization of the boil-spin method is warranted to permit remote use of the assay.
(© 2022. The Author(s).)
Databáze: MEDLINE
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