Genome-wide quantification of contributions to sexual fitness identifies genes required for spore viability and health in fission yeast.

Autor: Billmyre RB; Stowers Institute for Medical Research, Kansas City, Missouri, United States of America., Eickbush MT; Stowers Institute for Medical Research, Kansas City, Missouri, United States of America., Craig CJ; Stowers Institute for Medical Research, Kansas City, Missouri, United States of America., Lange JJ; Stowers Institute for Medical Research, Kansas City, Missouri, United States of America., Wood C; Stowers Institute for Medical Research, Kansas City, Missouri, United States of America., Helston RM; Stowers Institute for Medical Research, Kansas City, Missouri, United States of America., Zanders SE; Stowers Institute for Medical Research, Kansas City, Missouri, United States of America.; Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas, United States of America.
Jazyk: angličtina
Zdroj: PLoS genetics [PLoS Genet] 2022 Oct 27; Vol. 18 (10), pp. e1010462. Date of Electronic Publication: 2022 Oct 27 (Print Publication: 2022).
DOI: 10.1371/journal.pgen.1010462
Abstrakt: Numerous genes required for sexual reproduction remain to be identified even in simple model species like Schizosaccharomyces pombe. To address this, we developed an assay in S. pombe that couples transposon mutagenesis with high-throughput sequencing (TN-seq) to quantitatively measure the fitness contribution of nonessential genes across the genome to sexual reproduction. This approach identified 532 genes that contribute to sex, including more than 200 that were not previously annotated to be involved in the process, of which more than 150 have orthologs in vertebrates. Among our verified hits was an uncharacterized gene, ifs1 (important for sex), that is required for spore viability. In two other hits, plb1 and alg9, we observed a novel mutant phenotype of poor spore health wherein viable spores are produced, but the spores exhibit low fitness and are rapidly outcompeted by wild type. Finally, we fortuitously discovered that a gene previously thought to be essential, sdg1 (social distancing gene), is instead required for growth at low cell densities and can be rescued by conditioned medium. Our assay will be valuable in further studies of sexual reproduction in S. pombe and identifies multiple candidate genes that could contribute to sexual reproduction in other eukaryotes, including humans.
Competing Interests: I have read the journal’s policy and the authors of this manuscript have the following competing interests: SEZ: Inventor on patent application 834 serial 62/491,107 based on wtf killers.
(Copyright: © 2022 Billmyre et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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