3D Human iPSC Blood Vessel Organoids as a Source of Flow-Adaptive Vascular Cells for Creating a Human-Relevant 3D-Scaffold Based Macrovessel Model.

Autor: Meijer EM; Department of Nephrology and Hypertension, Division of Internal Medicine and Dermatology, University Medical Center Utrecht, 3584CX, Utrecht, The Netherlands.; Regenerative Medicine Center Utrecht, University Medical Center Utrecht, 3584CT, Utrecht, The Netherlands., Koch SE; Department of Biomedical Engineering, Eindhoven University of Technology, 5612AZ, Eindhoven, The Netherlands.; Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, 5612AZ, Eindhoven, The Netherlands., van Dijk CGM; Department of Nephrology and Hypertension, Division of Internal Medicine and Dermatology, University Medical Center Utrecht, 3584CX, Utrecht, The Netherlands.; Regenerative Medicine Center Utrecht, University Medical Center Utrecht, 3584CT, Utrecht, The Netherlands., Maas RGC; Regenerative Medicine Center Utrecht, Department of Cardiology, University Medical Center Utrecht, 3584CX, Utrecht, The Netherlands., Chrifi I; Department of Nephrology and Hypertension, Division of Internal Medicine and Dermatology, University Medical Center Utrecht, 3584CX, Utrecht, The Netherlands.; Regenerative Medicine Center Utrecht, University Medical Center Utrecht, 3584CT, Utrecht, The Netherlands., Szymczyk W; Department of Biomedical Engineering, Eindhoven University of Technology, 5612AZ, Eindhoven, The Netherlands., Besseling PJ; Department of Nephrology and Hypertension, Division of Internal Medicine and Dermatology, University Medical Center Utrecht, 3584CX, Utrecht, The Netherlands.; Regenerative Medicine Center Utrecht, University Medical Center Utrecht, 3584CT, Utrecht, The Netherlands., Pomp L; Department of Biomedical Engineering, Eindhoven University of Technology, 5612AZ, Eindhoven, The Netherlands., Koomen VJCH; Department of Biomedical Engineering, Eindhoven University of Technology, 5612AZ, Eindhoven, The Netherlands., Buikema JW; Regenerative Medicine Center Utrecht, Department of Cardiology, University Medical Center Utrecht, 3584CX, Utrecht, The Netherlands., Bouten CVC; Department of Biomedical Engineering, Eindhoven University of Technology, 5612AZ, Eindhoven, The Netherlands.; Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, 5612AZ, Eindhoven, The Netherlands., Verhaar MC; Department of Nephrology and Hypertension, Division of Internal Medicine and Dermatology, University Medical Center Utrecht, 3584CX, Utrecht, The Netherlands.; Regenerative Medicine Center Utrecht, University Medical Center Utrecht, 3584CT, Utrecht, The Netherlands., Smits AIPM; Department of Biomedical Engineering, Eindhoven University of Technology, 5612AZ, Eindhoven, The Netherlands.; Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, 5612AZ, Eindhoven, The Netherlands., Cheng C; Department of Nephrology and Hypertension, Division of Internal Medicine and Dermatology, University Medical Center Utrecht, 3584CX, Utrecht, The Netherlands.; Regenerative Medicine Center Utrecht, University Medical Center Utrecht, 3584CT, Utrecht, The Netherlands.; Experimental Cardiology, Department of Cardiology, Thoraxcenter Erasmus University Medical Center, 3015GD, Rotterdam, The Netherlands.
Jazyk: angličtina
Zdroj: Advanced biology [Adv Biol (Weinh)] 2023 Jan; Vol. 7 (1), pp. e2200137. Date of Electronic Publication: 2022 Oct 27.
DOI: 10.1002/adbi.202200137
Abstrakt: 3D-scaffold based in vitro human tissue models accelerate disease studies and screening of pharmaceutics while improving the clinical translation of findings. Here is reported the use of human induced pluripotent stem cell (hiPSC)-derived vascular organoid cells as a new cell source for the creation of an electrospun polycaprolactone-bisurea (PCL-BU) 3D-scaffold-based, perfused human macrovessel model. A separation protocol is developed to obtain monocultures of organoid-derived endothelial cells (ODECs) and mural cells (ODMCs) from hiPSC vascular organoids. Shear stress responses of ODECs versus HUVECs and barrier function (by trans endothelial electrical resistance) are measured. PCL-BU scaffolds are seeded with ODECs and ODMCs, and tissue organization and flow adaptation are evaluated in a perfused bioreactor system. ODECs and ODMCs harvested from vascular organoids can be cryopreserved and expanded without loss of cell purity and proliferative capacity. ODECs are shear stress responsive and establish a functional barrier that self-restores after the thrombin challenge. Static bioreactor culture of ODECs/ODMCs seeded scaffolds results in a biomimetic vascular bi-layer hierarchy, which is preserved under laminar flow similar to scaffolds seeded with primary vascular cells. HiPSC-derived vascular organoids can be used as a source of functional, flow-adaptive vascular cells for the creation of 3D-scaffold based human macrovascular models.
(© 2022 The Authors. Advanced Biology published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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