A highly polymorphic effector protein promotes fungal virulence through suppression of plant-associated Actinobacteria.

Autor: Snelders NC; Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, 50674, Cologne, Germany.; Theoretical Biology and Bioinformatics Group, Department of Biology, University of Utrecht, 3584CH, Utrecht, the Netherlands., Boshoven JC; Laboratory of Phytopathology, Wageningen University and Research, Droevendaalsesteeg 1, 6708PB, Wageningen, the Netherlands., Song Y; Laboratory of Phytopathology, Wageningen University and Research, Droevendaalsesteeg 1, 6708PB, Wageningen, the Netherlands.; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China., Schmitz N; Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, 50674, Cologne, Germany., Fiorin GL; Laboratory of Phytopathology, Wageningen University and Research, Droevendaalsesteeg 1, 6708PB, Wageningen, the Netherlands., Rovenich H; Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, 50674, Cologne, Germany., van den Berg GCM; Laboratory of Phytopathology, Wageningen University and Research, Droevendaalsesteeg 1, 6708PB, Wageningen, the Netherlands., Torres DE; Theoretical Biology and Bioinformatics Group, Department of Biology, University of Utrecht, 3584CH, Utrecht, the Netherlands.; Laboratory of Phytopathology, Wageningen University and Research, Droevendaalsesteeg 1, 6708PB, Wageningen, the Netherlands., Petti GC; Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, 50674, Cologne, Germany., Prockl Z; Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, 50674, Cologne, Germany., Faino L; Laboratory of Phytopathology, Wageningen University and Research, Droevendaalsesteeg 1, 6708PB, Wageningen, the Netherlands.; Department of Ambiental Biology, Sapienza University of Rome, 00185, Rome, Italy., Seidl MF; Theoretical Biology and Bioinformatics Group, Department of Biology, University of Utrecht, 3584CH, Utrecht, the Netherlands., Thomma BPHJ; Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, 50674, Cologne, Germany.; Laboratory of Phytopathology, Wageningen University and Research, Droevendaalsesteeg 1, 6708PB, Wageningen, the Netherlands.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2023 Feb; Vol. 237 (3), pp. 944-958. Date of Electronic Publication: 2022 Nov 29.
DOI: 10.1111/nph.18576
Abstrakt: Plant pathogens secrete effector proteins to support host colonization through a wide range of molecular mechanisms, while plant immune systems evolved receptors to recognize effectors or their activities to mount immune responses to halt pathogens. Importantly, plants do not act as single organisms, but rather as holobionts that actively shape their microbiota as a determinant of health. The soil-borne fungal pathogen Verticillium dahliae was recently demonstrated to exploit the VdAve1 effector to manipulate the host microbiota to promote vascular wilt disease in the absence of the corresponding immune receptor Ve1. We identify a multiallelic V. dahliae gene displaying c. 65% sequence similarity to VdAve1, named VdAve1-like (VdAve1L), which shows extreme sequence variation, including alleles that encode dysfunctional proteins, indicative of selection pressure to overcome host recognition. We show that the orphan cell surface receptor Ve2, encoded at the Ve locus, does not recognize VdAve1L. Additionally, we demonstrate that the full-length variant VdAve1L2 possesses antimicrobial activity, like VdAve1, yet with a divergent activity spectrum, that is exploited by V. dahliae to mediate tomato colonization through the direct suppression of antagonistic Actinobacteria in the host microbiota. Our findings open up strategies for more targeted biocontrol against microbial plant pathogens.
(© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.)
Databáze: MEDLINE
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