Evaluation of new Toxocara canis chimeric antigens as an alternative to conventional TES-Ag for anti- Toxocara antibodies detection.

Autor:	Mesa-Arango JA; Centro Nacional de Secuenciación Genómica-CNSG, Sede de Investigación Universitaria-SIU, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Colombia.; Grupo de Parasitología, Facultad de Medicina, Universidad de Antioquia, Colombia., Olave-Velandia AM; Centro Nacional de Secuenciación Genómica-CNSG, Sede de Investigación Universitaria-SIU, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Colombia., García-Montoya GM; Centro Nacional de Secuenciación Genómica-CNSG, Sede de Investigación Universitaria-SIU, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Colombia.; Grupo de Parasitología, Facultad de Medicina, Universidad de Antioquia, Colombia., Isaza-Agudelo JP; Centro Nacional de Secuenciación Genómica-CNSG, Sede de Investigación Universitaria-SIU, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Colombia.; Grupo de Parasitología, Facultad de Medicina, Universidad de Antioquia, Colombia., Jiménez-Ruiz A; Departamento de Biología de Sistemas, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain., Alzate JF; Centro Nacional de Secuenciación Genómica-CNSG, Sede de Investigación Universitaria-SIU, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Colombia.; Grupo de Parasitología, Facultad de Medicina, Universidad de Antioquia, Colombia.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2022 Oct 18; Vol. 8 (10), pp. e11144. Date of Electronic Publication: 2022 Oct 18 (Print Publication: 2022).
DOI:	10.1016/j.heliyon.2022.e11144
Abstrakt:	Human toxocariasis is one of the neglected helminthiases and it is caused by the zoonotic roundworm species Toxocara canis and Toxocara cati . Diagnosis of human toxocariasis is based on the combination of clinical, parasitological, and epidemiological criteria, as well as serology tests that detect anti- Toxocara antibodies. Notwithstanding, due to the absence of pathognomonic symptoms and signs of the disease, serology is the key evidence to support a conclusive diagnosis. TES-ELISA is the most widely used serological test for diagnosis. However, cross-reaction of TES antigens with antibodies produced to other helminth antigens is a major drawback for its application in countries with high parasitic prevalence. T. canis recombinant antigens have been described as an alternative to native TES for diagnosis. Nevertheless, the selection of antigenic proteins is a complex process that requires validation. In this paper, we developed an eGFP carrier-based system to express and purify blocks of recombinant polypeptides of T. canis antigenic proteins. Intense cross-reaction polypeptides were detected by Immunoblot and avoided to finally produce a chimeric prototype protein. Additionally, a control chimeric protein that harbors the complete tested proteins was produced. Purified chimeric antigens were tested in ELISA and Immunoblot assays with 310 sera samples of negative and positive control individuals. Our results showed that chimeric rCHITC0 and rCHITC1 antigens (with sensitivities of 62% 58%, 38% and 16% in IB-rCHITC0, ELISA-rCHITC0, ELISA-rCHITC1 and IB-rCHITC1 respectively for OLMS) can perform better in terms of specificity (being 91%, 89%, 87% and 76% for ELISA-rCHITC1, IB-rCHITC1, ELISA-rCHITC0 and IB-rCHITC0 respectively for OLMS) than T. canis TES-ELISA (with 61% specificity), giving a higher signal with serum samples of infected individuals as well the possibility to discriminate false positive cases with other parasitic infections. Our data suggest that T. canis chimeric proteins, represent candidate antigens for phase II studies. Competing Interests: The authors declare no conflict of interest. (© 2022 The Author(s).)
Databáze:	MEDLINE
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