Expression of microRNAs miR-21 and miR-326 associated with HIF-1α regulation in neurospheres of glioblastoma submitted to ionizing radiation treatment.

Autor:	Turra LP; Ribeirão Preto Medical School, University of São Paulo, Department of Surgery and Anatomy Ribeirão Preto - São Paulo, Brazil., Rodrigues AR; Ribeirão Preto Medical School, University of São Paulo, Department of Surgery and Anatomy Ribeirão Preto - São Paulo, Brazil., Neto FSL; Ribeirão Preto Medical School, University of São Paulo, Department of Surgery and Anatomy Ribeirão Preto - São Paulo, Brazil., Novais PC; Ribeirão Preto Medical School, University of São Paulo, Department of Surgery and Anatomy Ribeirão Preto - São Paulo, Brazil.; Postgraduate Program in Structural and Functional Interations in Rehabilitation, University of Marília, Marília - São Paulo, Brazil., Nunes MJ; Ribeirão Preto Medical School, University of São Paulo, Department of Surgery and Anatomy Ribeirão Preto - São Paulo, Brazil., Tirapelli VC; Ribeirão Preto Medical School, University of São Paulo, Department of Surgery and Anatomy Ribeirão Preto - São Paulo, Brazil., Peria FM; Ribeirão Preto Medical School, University of São Paulo, Department of Surgery and Anatomy Ribeirão Preto - São Paulo, Brazil., Carneiro VM; Ribeirão Preto Medical School, University of São Paulo, Department of Surgery and Anatomy Ribeirão Preto - São Paulo, Brazil., de Assis Cirino ML; Ribeirão Preto Medical School, University of São Paulo, Department of Surgery and Anatomy Ribeirão Preto - São Paulo, Brazil.; Anhanguera College of Ribeirão Preto, Ribeirão Preto - São Paulo, Brazil., Carlotti CG Jr; Ribeirão Preto Medical School, University of São Paulo, Department of Surgery and Anatomy Ribeirão Preto - São Paulo, Brazil., Tirapelli DPDC; Ribeirão Preto Medical School, University of São Paulo, Department of Surgery and Anatomy Ribeirão Preto - São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Reports of practical oncology and radiotherapy : journal of Greatpoland Cancer Center in Poznan and Polish Society of Radiation Oncology [Rep Pract Oncol Radiother] 2022 May 19; Vol. 27 (2), pp. 215-225. Date of Electronic Publication: 2022 May 19 (Print Publication: 2022).
DOI:	10.5603/RPOR.a2022.0040
Abstrakt:	Background: Glioblastoma is an incurable neoplasm. Its hypoxia mechanism associated with cancer stem cells (CSCs) demonstrates hypoxia-inducible factor 1α (HIF-1α) expression regulation, which is directly related to tumor malignancy. The aim of this study was to identify a possible tumor malignancy signature associated with regulation of HIF-1α by microRNAs miR-21 and miR-326 in the subpopulation of tumor stem cells which were irradiated by ion in primary culture of patients diagnosed with glioblastoma. Materials and Methods: We used cellular cultures from surgery biopsies of ten patients with glioblastoma. MicroRNA expressions were analyzed through real-time polymerase chain reaction (PCR ) and correlated with mortality and recurrence. The ROC curve displayed the cutoff point of the respective microRNAs in relation to the clinical prognosis, separating them by group. Results: The miR-21 addressed high level of expression in the irradiated neurosphere group (p = 0.0028). However, miR-21 was not associated with recurrence and mortality. miR-326 can be associated with tumoral recurrence (p = 0.032) in both groups; every 0.5 units of miR-326 increased the chances of recurrence by 1,024 (2.4%). Conclusion: The high expression of miR-21 in the irradiated group suggests its role in the regulation of HIF-1α and in the radioresistant neurospheres. miR-326 increased the chances of recurrence in both groups, also demonstrating that positive regulation from miR-326 does not depend on ionizing radiation treatment. Competing Interests: Conflict of interest The authors declare that they have no conflict of interest. (© 2022 Greater Poland Cancer Centre.)
Databáze:	MEDLINE
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