| Autor: |
Castañeda-Reyes ED; Vegetal Proteins Laboratoy, Department of Biochemical Engineering, National School of Biological Sciences, National Polytechnic Institute, (IPN), Av. Wilfrido Massieu, and Miguel Stampa s/n, Zacatenco, Gustavo A. Madero, Mexico City 07738, Mexico.; National Center of Nanosciences and Micro and Nanotechnologies, National Polytechnic Institute (IPN), Av. Luis Enrique Erro s/n, Unidad Profesional Adolfo López Mateos, Zacatenco, Alcaldía Gustavo A. Madero, Mexico City 07738, Mexico.; Department of Food Science and Human Nutrition, 228 ER Madigan Laboratory, University of Illinois, 2101 W. Gregory Dr, Champaign, IL 61801, USA., Perea-Flores MJ; National Center of Nanosciences and Micro and Nanotechnologies, National Polytechnic Institute (IPN), Av. Luis Enrique Erro s/n, Unidad Profesional Adolfo López Mateos, Zacatenco, Alcaldía Gustavo A. Madero, Mexico City 07738, Mexico., Dávila-Ortiz G; Vegetal Proteins Laboratoy, Department of Biochemical Engineering, National School of Biological Sciences, National Polytechnic Institute, (IPN), Av. Wilfrido Massieu, and Miguel Stampa s/n, Zacatenco, Gustavo A. Madero, Mexico City 07738, Mexico., Gonzalez de Mejia E; Department of Food Science and Human Nutrition, 228 ER Madigan Laboratory, University of Illinois, 2101 W. Gregory Dr, Champaign, IL 61801, USA. |
| Abstrakt: |
The objective of this study was to assess the effectiveness of liposomes loaded with soybean lunasin and amaranth unsaponifiable matter (UM + LunLip) as a source of squalene in the prevention of melanoma skin cancer in an allograft mice model. Tumors were induced by transplanting melanoma B16-F10 cells into the mice. The most effective treatments were those including UM + LunLip, with no difference between the lunasin concentrations (15 or 30 mg/kg body weight); however, these treatments were statistically different from the tumor-bearing untreated control (G3) (p < 0.05). The groups treated with topical application showed significant inhibition (68%, p < 0.05) compared to G3. The groups treated with subcutaneous injections showed significant inhibition (up to 99%, p < 0.05) in G3. During tumor development, UM + LunLip treatments under-expressed Ki-67 (0.2-fold compared to G3), glycogen synthase kinase-3β (0.1-fold compared to G3), and overexpressed caspase-3 (30-fold compared to G3). In addition, larger tumors showed larger necrotic areas (38% with respect to the total tumor) (p < 0.0001). In conclusion, the UM + LunLip treatment was effective when applied either subcutaneously or topically in the melanoma tumor-developing groups, as it slowed down cell proliferation and activated apoptosis. |
