| Autor: |
de Menezes SAF; School of Dentistry, University Center of State of Pará, Belém 66060-575, PA, Brazil., Miranda YMS; School of Dentistry, University Center of State of Pará, Belém 66060-575, PA, Brazil., da Silva YM; School of Dentistry, University Center of State of Pará, Belém 66060-575, PA, Brazil., Carvalho TRB; School of Dentistry, University Center of State of Pará, Belém 66060-575, PA, Brazil., Alves FRS; Evandro Chagas Institute, Health Ministry of Brazil, Ananindeua 67030-000, PA, Brazil., Silvestre RVD; Evandro Chagas Institute, Health Ministry of Brazil, Ananindeua 67030-000, PA, Brazil., Oliveira-Filho AB; Study and Research Group on Vulnerable Populations, Institute for Coastal Studies, Federal University of Pará, Bragança 68600-000, PA, Brazil., de Alencar Menezes TO; School of Dentistry, Federal University of Pará, Belém 66075-110, PA, Brazil., de Souza Fonseca RR; Biology of Infectious and Parasitic Agents Post-Graduate Program, Federal University of Pará, Belém 66075-110, PA, Brazil.; Virology Laboratory, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, PA, Brazil., Laurentino RV; Virology Laboratory, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, PA, Brazil., Machado LFA; Biology of Infectious and Parasitic Agents Post-Graduate Program, Federal University of Pará, Belém 66075-110, PA, Brazil.; Virology Laboratory, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, PA, Brazil. |
| Abstrakt: |
Highly oncogenic human papillomavirus (HPV) is well known to be associated with and a risk factor for various types of oral carcinomas such as oral squamous cell carcinoma (OSCC). The aim of this study was to evaluate and describe the HPV-induced OSCC prevalence and genotyping in the city of Belém, northern Brazil. This cross-sectional study features 101 participants who attended an oral pathology referral center in a dental college looking for diagnoses of oral lesions (OL). After signing the consent term and meeting the inclusion criteria, all participants went through a sociodemographic and epidemiological questionnaire. Then, OL were collected by excisional or incisional biopsy depending on OL size; after that, OL tissues were preserved in paraffin blocks to histopathological diagnoses. Afterwards, paraffin blocks were divided into benign and malignant/premalignant lesions based on the classification of potentially malignant disorders of the oral and oropharyngeal mucosa. Then, the paraffin blocks had DNA extraction performed by the ReliaPrep FFPE gDNA Miniprep method in order to identify HPV DNA of high oncogenic risk and low oncogenic risk. Then, the viral DNA was amplified and typed using the Inno-Lipa genotyping Extra II method, and the collected data were analyzed by Chi-square and G-tests. In total, 59/101 (58.4%) OL were malignant/premalignant lesions, of which OSCC was the most prevalent with 40/59 (67.7%) and 42/101 (41.6%) benign lesions. The most common area of OL incidence was upper gingiva 46/101 (45.5%). Regarding HPV DNA detection, approximately 27/101 (26.7%) had positive results; of these, 17/59 (28.8%) were malignant/premalignant lesions, and the most prevalent genotypes detected were 16, 18, 52 and 58, while among benign lesions, 10/42 (66.6%) had HPV-positive results, and the most prevalent genotypes detected were 6, 11 and 42. Age range was the only risk factor with a significant association between HPV and OSCC presence ( p -value: 0.0004). A correlation between OSCC and oral HPV among analyzed samples could not be demonstrated in our small cohort. |
