Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma.

Autor: Mello VC; Postgraduate Program in Animal Biology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasília, Brasília 70910-900, DF, Brazil.; Laboratory of Microscopy and Microanalysis, Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília 70910-900, DF, Brazil.; COOIL Institute, Brasília 72622-401, DF, Brazil., Araújo VHS; School of Pharmaceutical Sciences, São Paulo State University, Araraquara 14800-903, SP, Brazil., de Paiva KLR; Laboratory of Microscopy and Microanalysis, Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília 70910-900, DF, Brazil.; COOIL Institute, Brasília 72622-401, DF, Brazil., Simões MM; Laboratory of Microscopy and Microanalysis, Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília 70910-900, DF, Brazil.; COOIL Institute, Brasília 72622-401, DF, Brazil., Marques DC; Laboratory of Microscopy and Microanalysis, Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília 70910-900, DF, Brazil.; COOIL Institute, Brasília 72622-401, DF, Brazil., da Silva Costa NR; Laboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasília 70910-900, DF, Brazil., de Souza IF; Laboratory of Microscopy and Microanalysis, Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília 70910-900, DF, Brazil., da Silva PB; Laboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasília 70910-900, DF, Brazil., Santos I; Laboratory of Immunology and Inflammation, Department of Cell Biology, University of Brasilia, Brasília 70910-900, DF, Brazil., Almeida R; Laboratory of Immunology and Inflammation, Department of Cell Biology, University of Brasilia, Brasília 70910-900, DF, Brazil., Magalhães KG; Laboratory of Immunology and Inflammation, Department of Cell Biology, University of Brasilia, Brasília 70910-900, DF, Brazil., da Silva SW; Optical Spectroscopy Laboratory, Institute of Physics, University of Brasilia, Brasília 70910-900, DF, Brazil., Santos AS; Optical Spectroscopy Laboratory, Institute of Physics, University of Brasilia, Brasília 70910-900, DF, Brazil., Veiga-Souza F; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasília, Brasília 70910-900, DF, Brazil.; Faculty of Ceilandia, University of Brasilia, Brasília 70910-900, DF, Brazil., Souza PEN; Laboratory of Electron Paramagnetic Resonance, Institute of Physics, University of Brasília, Brasília 70910-900, DF, Brazil., Raddichi MA; Laboratory of Microscopy and Microanalysis, Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília 70910-900, DF, Brazil.; Laboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasília 70910-900, DF, Brazil., Longo JPF; Laboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasília 70910-900, DF, Brazil., de Araújo JTC; School of Pharmaceutical Sciences, São Paulo State University, Araraquara 14800-903, SP, Brazil., Muehlmann LA; Faculty of Ceilandia, University of Brasilia, Brasília 70910-900, DF, Brazil., Chorilli M; School of Pharmaceutical Sciences, São Paulo State University, Araraquara 14800-903, SP, Brazil., Báo SN; Laboratory of Microscopy and Microanalysis, Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília 70910-900, DF, Brazil.
Jazyk: angličtina
Zdroj: Nanomaterials (Basel, Switzerland) [Nanomaterials (Basel)] 2022 Oct 11; Vol. 12 (20). Date of Electronic Publication: 2022 Oct 11.
DOI: 10.3390/nano12203547
Abstrakt: Photodynamic therapy (PDT) mediated by photosensitizers loaded in nanostructures as solid lipid nanoparticles has been pinpointed as an effective and safe treatment against different skin cancers. Amazon butters have an interesting lipid composition when it comes to forming solid lipid nanoparticles (SLN). In the present report, a new third-generation photosensitizing system consisting of aluminum-phthalocyanine associated with Amazon butter-based solid lipid nanoparticles (SLN-AlPc) is described. The SLN was developed using murumuru butter, and a monodisperse population of nanodroplets with a hydrodynamic diameter of approximately 40 nm was obtained. The study of the permeation of these AlPc did not permeate the analyzed skin, but when incorporated into the system, SLN-AlPc allowed permeation of almost 100% with 8 h of contact. It must be emphasized that SLN-AlPc was efficient for carrying aluminum-phthalocyanine photosensitizers and exhibited no toxicity in the dark. Photoactivated SLN-AlPc exhibited a 50% cytotoxicity concentration (IC 50 ) of 19.62 nM when applied to B16-F10 monolayers, and the type of death caused by the treatment was apoptosis. The exposed phospholipid phosphatidylserine was identified, and the treatment triggered a high expression of Caspase 3. A stable Amazon butter-based SLN-AlPc formulation was developed, which exhibits strong in vitro photodynamic activity on melanoma cells.
Databáze: MEDLINE