| Autor: |
Navarro A; Public Health Department, Faculty of Medicine, National Autonomous University of Mexico, Av. Universidad 3000, Ciudad Universitaria, Mexico City 04510, Mexico., Rodea GE; Bacterial Pathogenicity Laboratory, Hemato-Oncology Research Unit, Federico Gómez Children's Hospital of Mexico/Faculty of Medicine UNAM, Mexico City 06720, Mexico., Castelán-Sánchez HG; Researchers for Mexico Program, Genomics and Evolutionary Dynamics Group of Emerging Microorganisms, National Council of Science and Technology, (CONACYT), Mexico City 82996, Mexico., Saucedo-Pastrana HA; Public Health Services of Mexico City, Mexico City 04730, Mexico., Licona-Moreno D; Public Health Department, Faculty of Medicine, National Autonomous University of Mexico, Av. Universidad 3000, Ciudad Universitaria, Mexico City 04510, Mexico., Eslava-Campos C; Public Health Department, Faculty of Medicine, National Autonomous University of Mexico, Av. Universidad 3000, Ciudad Universitaria, Mexico City 04510, Mexico.; Peripheral Unit of Basic and Clinical Research in Infectious Diseases, Hemato-Oncology and Research Building, Children's Hospital of Mexico Federico Gómez, Dr. Márquez 162, Col. De los Doctores, Mexico City 06720, Mexico., Tirado-Gómez LL; Public Health Department, Faculty of Medicine, National Autonomous University of Mexico, Av. Universidad 3000, Ciudad Universitaria, Mexico City 04510, Mexico., Vilchis-Reyes A; Public Health Department, Faculty of Medicine, National Autonomous University of Mexico, Av. Universidad 3000, Ciudad Universitaria, Mexico City 04510, Mexico., García de la Torre G; Public Health Department, Faculty of Medicine, National Autonomous University of Mexico, Av. Universidad 3000, Ciudad Universitaria, Mexico City 04510, Mexico., Cruz-Licea V; Public Health Department, Faculty of Medicine, National Autonomous University of Mexico, Av. Universidad 3000, Ciudad Universitaria, Mexico City 04510, Mexico. |
| Abstrakt: |
Antimicrobial resistance (AMR) is a relevant public health problem worldwide, and microbiome bacteria may contribute to the horizontal gene transfer associated with antimicrobial resistance. The microbiome of fecal samples from Mexican adolescents were analyzed and correlated with eating habits, and the presence of AMR genes on bacteria in the microbiome was evaluated. Fecal samples from adolescents were collected and processed to extract genomic DNA. An Illumina HiSeq 1500 system was used to determine resistance genes and the microbiome of adolescents through the amplification of gene resistance and the V3-V4 regions of RNA, respectively. Analysis of the microbiome from fecal samples taken from 18 obese, overweight, and normal-weight adolescents revealed that the Firmicutes was the most frequent phylum, followed by Bacteroidetes, Actinobacteria, Proteobacteria and Verrucomicrobia. The following species were detected as the most frequent in the samples: F. prausnitzii , P. cori , B. adolescentis , E. coli and A. muciniphila . The presence of Bacteroides , Prevotella and Ruminococcus was used to establish the enterotype; enterotype 1 was more common in women and enterotype 2 was more common in men. Twenty-nine AMR genes were found for β-lactamases, fluoroquinolones, aminoglycosides, macrolide, lincosamides, streptogramin (MLS), tetracyclines and sulfonamides. The presence of microorganisms in fecal samples that harbor AMR genes that work against antimicrobials frequently used for the treatment of microbial infections such as b-lactams, macrolides, aminoglycosides, MLS, and tetracyclines is of great concern, as these organisms may be an important reservoir for horizontal AMR gene transfer. |
