| Autor: |
Ashry M; Zoology Department, Faculty of Science, Al-Azhar University, Assiut 71524, Egypt., Askar H; Zoology Department, Faculty of Science, Al-Azhar University, Assiut 71524, Egypt., Alian A; Zoology Department, Faculty of Science, Al-Azhar University, Assiut 71524, Egypt., Zidan SAH; Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, Egypt., El-Sahra DG; Medical Surgical Nursing Department, Faculty of Nursing, Modern University for Technology and Information, Cairo 12055, Egypt., Abdel-Wahhab KG; Medical Physiology Department, National Research Centre, Giza 12622, Egypt., Lamlom SF; Plant Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt., Abdelsalam NR; Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt., Abd El-Hack ME; Department of Poultry, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt., Gomaa HF; Department of Zoology, Faculty of Science, Ain-Shams University, Cairo 11566, Egypt. |
| Abstrakt: |
One of the most common tumors to cause death worldwide is colon cancer. This study aims to investigate the antitumor potency of Litophyton sp. methanolic extract (LME) against DMH-induced colon cancer in adult male rats. Group (1) normal rats served as the control, group (2) normal rats were ip-injected with LME at a dose of 100 μg/kg/day, group (3) DMH-induced colon cancer animals, and group (4) colon cancer-modeled animals were treated with LME (100 μg/kg/day) for six weeks. The results revealed that injection of LME markedly regenerated the colon cancer pathophysiological disorders; this was monitored from the significant reduction in the values of serum biomarkers (CEA, CA19.9, AFP), cytokines (TNF-α and IL1β), and biochemical measurements (ALAT, ASAT, urea, creatinine, cholesterol, and triglycerides) matched significant increase of apoptotic biomarkers (CD4+); similarly, colon DNA fragmentation, MDA, and NO levels were down-regulated. In contrast, a remarkable upregulation in colon SOD, GPx, GSH, and CAT levels was noted. Moreover, the colon histopathological architecture showed obvious regenerations. Chromatography of LME resulted in the purification of two polyhydroxylated steroids (1 and 2) with potential cytotoxic activities. LME performed therapeutic potential colon tumorigenesis; therefore, LME may have a promising chemo-preventive feature against colon cancer, probably via enhancement of the apoptosis pathway, improvement of the immune response, reduction of inflammation, or/and restoration of the impaired oxidative stress. |
