| Autor: |
Nargesi S; Department of Medical Mycology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari 4815733971, Iran.; Invasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari 4815733971, Iran., Valadan R; Department of Immunology/Molecular and Cell Biology Research Center (MCBRC), Mazandaran University of Medical Sciences, Sari 48175-1665, Iran., Abastabar M; Department of Medical Mycology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari 4815733971, Iran.; Invasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari 4815733971, Iran., Kaboli S; Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan 4513956111, Iran.; Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan 4513956111, Iran., Thekkiniath J; Fuller Laboratories, 1312 East Valencia Drive, Fullerton, CA 92831, USA., Hedayati MT; Department of Medical Mycology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari 4815733971, Iran.; Invasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari 4815733971, Iran. |
| Abstrakt: |
The antifungal resistance in non-fumigatus Aspergillus spp., as well as Aspergillus fumigatus , poses a major therapeutic challenge which affects the entire healthcare community. Mutation occurrence of cyp 51 gene paralogs is the major cause of azole resistance in Aspergillus spp. To obtain a full map of genomic changes, an accurate scan of the entire length of the Aspergillus genome is necessary. In this study, using whole genome sequencing (WGS) technique, we evaluated the mutation in cyp51A , cyp51B , Cdr1B, AtrR, Hmg1, HapE and FfmA genes in different clinical isolates of Aspergillus fumigatus , Aspergillus niger , Aspergillus tubingensis , Aspergillus welwitschiae and Aspergillus terreus which responded to minimum inhibitory concentrations of itraconazole above 16 µg mL -1 . We found different nonsynonymous mutations in the cyp51A , cyp51B , Cdr1B , AtrR, Hmg1, HapE and FfmA gene loci. According to our findings, Aspergillus species isolated from different parts of the world may represent different pattern of resistance mechanisms which may be revealed by WGS. |
