| Autor: |
Dementieva NV; Russian Research Institute of Farm Animal Genetics and Breeding-Branch of the L.K. Ernst Federal Research Centre for Animal Husbandry, Pushkin, 196601 St. Petersburg, Russia., Shcherbakov YS; Russian Research Institute of Farm Animal Genetics and Breeding-Branch of the L.K. Ernst Federal Research Centre for Animal Husbandry, Pushkin, 196601 St. Petersburg, Russia., Tyshchenko VI; Russian Research Institute of Farm Animal Genetics and Breeding-Branch of the L.K. Ernst Federal Research Centre for Animal Husbandry, Pushkin, 196601 St. Petersburg, Russia., Terletsky VP; Pushkin Leningrad State University, Pushkin, 196605 St. Petersburg, Russia., Vakhrameev AB; Russian Research Institute of Farm Animal Genetics and Breeding-Branch of the L.K. Ernst Federal Research Centre for Animal Husbandry, Pushkin, 196601 St. Petersburg, Russia., Nikolaeva OA; Russian Research Institute of Farm Animal Genetics and Breeding-Branch of the L.K. Ernst Federal Research Centre for Animal Husbandry, Pushkin, 196601 St. Petersburg, Russia., Ryabova AE; Russian Research Institute of Farm Animal Genetics and Breeding-Branch of the L.K. Ernst Federal Research Centre for Animal Husbandry, Pushkin, 196601 St. Petersburg, Russia., Azovtseva AI; Russian Research Institute of Farm Animal Genetics and Breeding-Branch of the L.K. Ernst Federal Research Centre for Animal Husbandry, Pushkin, 196601 St. Petersburg, Russia., Mitrofanova OV; Russian Research Institute of Farm Animal Genetics and Breeding-Branch of the L.K. Ernst Federal Research Centre for Animal Husbandry, Pushkin, 196601 St. Petersburg, Russia., Peglivanyan GK; Russian Research Institute of Farm Animal Genetics and Breeding-Branch of the L.K. Ernst Federal Research Centre for Animal Husbandry, Pushkin, 196601 St. Petersburg, Russia., Reinbah NR; Russian Research Institute of Farm Animal Genetics and Breeding-Branch of the L.K. Ernst Federal Research Centre for Animal Husbandry, Pushkin, 196601 St. Petersburg, Russia., Griffin DK; School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK., Romanov MN; School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK. |
| Abstrakt: |
Monitoring the genetic diversity of small populations is important with respect to conserving rare and valuable chicken breeds, as well as discovery and innovation in germplasm research and application. Restriction fragment length polymorphisms (RFLPs), the molecular markers that underlie multilocus DNA fingerprinting (MLDF), have historically been employed for this purpose, but over the past two decades, there has been an irreversible shift toward high-throughput single-nucleotide polymorphisms (SNPs). In this study, we conducted a comparative analysis of archived MLDF results and new data from whole-genome SNP genotyping (SNPg) among 18 divergently selected breeds representing a large sample of the world gene pool. As a result, we obtained data that fit the general concept of the phylogenetic distribution of the studied breeds and compared them with RFLP and SNP markers. RFLPs were found to be useful markers for retrospective assessment of changes in the genetic architecture and variability underlying the phenotypic variation in chicken populations, especially when samples from previous generations used for MLDF are unavailable for SNPg. These results can facilitate further research necessary to assess the possibility of extrapolating previous MLDF results to study the long-term dynamics of genetic diversity in various small chicken germplasm populations over time. In general, the whole-genome characterization of populations and breeds by multiple SNP loci will further form the basis for the development and implementation of genomic selection with the aim of effective use of the genetic potential of the domestic gene pool in the poultry industry. |
