| Autor: |
Farhan A; Department of Chemistry, Chung Yuan Christian University, Taoyuan 320314, Taiwan.; Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 320314, Taiwan., Saputra F; Department of Chemistry, Chung Yuan Christian University, Taoyuan 320314, Taiwan.; Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 320314, Taiwan., Suryanto ME; Department of Chemistry, Chung Yuan Christian University, Taoyuan 320314, Taiwan.; Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 320314, Taiwan., Humayun F; Department of Bioinformatics and Biostatistics, Shanghai Jiao Tong University, Shanghai 200240, China., Pajimna RMB; Faculty of Pharmacy, University of Santo Tomas, Manila 1015, Philippines.; The Graduate School, University of Santo Tomas, Manila 1015, Philippines., Vasquez RD; Faculty of Pharmacy, University of Santo Tomas, Manila 1015, Philippines.; The Graduate School, University of Santo Tomas, Manila 1015, Philippines.; Research Center for the Natural and Applied Sciences, University of Santo Tomas, Manila 1015, Philippines., Roldan MJM; Faculty of Pharmacy, University of Santo Tomas, Manila 1015, Philippines.; The Graduate School, University of Santo Tomas, Manila 1015, Philippines., Audira G; Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 320314, Taiwan., Lai HT; Department of Aquatic Biosciences, National Chiayi University, 300 University Rd., Chiayi 60004, Taiwan., Lai YH; Department of Chemistry, Chinese Culture University, Taipei 11114, Taiwan., Hsiao CD; Department of Chemistry, Chung Yuan Christian University, Taoyuan 320314, Taiwan.; Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 320314, Taiwan.; Research Center for Aquatic Toxicology and Pharmacology, Chung Yuan Christian University, Taoyuan 320314, Taiwan. |
| Abstrakt: |
The transparent appearance of fish embryos provides an excellent assessment feature for observing cardiovascular function in vivo. Previously, methods to conduct vascular function assessment were based on measuring blood-flow velocity using third-party software. In this study, we reported a simple software, free of costs and skills, called OpenBloodFlow, which can measure blood flow velocity and count blood cells in fish embryos for the first time. First, videos captured by high-speed CCD were processed for better image stabilization and contrast. Next, the optical flow of moving objects was extracted from the non-moving background in a frame-by-frame manner. Finally, blood flow velocity was calculated by the Gunner Farneback algorithm in Python. Data validation with zebrafish and medaka embryos in OpenBloodFlow was consistent with our previously published ImageJ-based method. We demonstrated consistent blood flow alterations by either OpenBloodFlow or ImageJ in the dorsal aorta of zebrafish embryos when exposed to either phenylhydrazine or ractopamine. In addition, we validated that OpenBloodFlow was able to conduct precise blood cell counting. In this study, we provide an easy and fully automatic programming for blood flow velocity calculation and blood cell counting that is useful for toxicology and pharmacology studies in fish. |
