Limosilactobacillus fermentum Strain 3872: Antibacterial and Immunoregulatory Properties and Synergy with Prebiotics against Socially Significant Antibiotic-Resistant Infections of Animals and Humans.

Autor: Abramov VM; Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor), Federal State Budgetary Institution 'The Russian State Center for Animal Feed and Drug Standardization and Quality' (FGBU VGNKI), 123022 Moscow, Russia.; Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia., Kosarev IV; Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor), Federal State Budgetary Institution 'The Russian State Center for Animal Feed and Drug Standardization and Quality' (FGBU VGNKI), 123022 Moscow, Russia.; Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia., Machulin AV; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center 'Pushchino Scientific Center for Biological Research of Russian Academy of Science', Russian Academy of Science, 142290 Pushchino, Russia., Priputnevich TV; Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia., Chikileva IO; Laboratory of Cell Immunity, Blokhin National Research Center of Oncology, Ministry of Health RF, 115478 Moscow, Russia., Deryusheva EI; Institute for Biological Instrumentation, Federal Research Center 'Pushchino Scientific Center for Biological Research of Russian Academy of Science', Russian Academy of Science, 142290 Pushchino, Russia., Abashina TN; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center 'Pushchino Scientific Center for Biological Research of Russian Academy of Science', Russian Academy of Science, 142290 Pushchino, Russia., Donetskova AD; NRC Institute of Immunology FMBA of Russia, 115522 Moscow, Russia., Panin AN; Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor), Federal State Budgetary Institution 'The Russian State Center for Animal Feed and Drug Standardization and Quality' (FGBU VGNKI), 123022 Moscow, Russia., Melnikov VG; Gabrichevsky Research Institute for Epidemiology and Microbiology, 125212 Moscow, Russia., Suzina NE; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center 'Pushchino Scientific Center for Biological Research of Russian Academy of Science', Russian Academy of Science, 142290 Pushchino, Russia., Nikonov IN; Federal State Educational Institution of Higher Professional Education, Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin, 109472 Moscow, Russia., Selina MV; Federal State Educational Institution of Higher Professional Education, Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin, 109472 Moscow, Russia., Khlebnikov VS; Institute of Immunological Engineering, 142380 Lyubuchany, Russia., Sakulin VK; Institute of Immunological Engineering, 142380 Lyubuchany, Russia., Vasilenko RN; Institute of Immunological Engineering, 142380 Lyubuchany, Russia., Samoilenko VA; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center 'Pushchino Scientific Center for Biological Research of Russian Academy of Science', Russian Academy of Science, 142290 Pushchino, Russia., Uversky VN; Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA., Karlyshev AV; Department of Biomolecular Sciences, Faculty of Health, Science, Social Care and Education, Kingston University London, Kingston upon Thames KT1 2EE, UK.
Jazyk: angličtina
Zdroj: Antibiotics (Basel, Switzerland) [Antibiotics (Basel)] 2022 Oct 19; Vol. 11 (10). Date of Electronic Publication: 2022 Oct 19.
DOI: 10.3390/antibiotics11101437
Abstrakt: Limosilactobacillus fermentum strain 3872 (LF3872) was originally isolated from the breast milk of a healthy woman during lactation and the breastfeeding of a child. The high-quality genome sequencing of LF3872 was performed, and a gene encoding a unique bacteriocin was discovered. It was established that the bacteriocin produced by LF3872 (BLF3872) belongs to the family of cell-wall-degrading proteins that cause cell lysis. The antibacterial properties of LF3872 were studied using test cultures of antibiotic-resistant Gram-positive and Gram-negative pathogens. Gram-positive pathogens ( Staphylococcus aureus strain 8325-4 and S. aureus strain IIE CI-SA 1246) were highly sensitive to the bacteriolytic action of LF3872. Gram-negative pathogens ( Escherichia coli , Salmonella strains, and Campylobacter jejuni strains) were more resistant to the bacteriolytic action of LF3872 compared to Gram-positive pathogens. LF3872 is a strong co-aggregator of Gram-negative pathogens. The cell-free culture supernatant of LF3872 (CSLF3872) induced cell damage in the Gram-positive and Gram-negative test cultures and ATP leakage. In the in vitro experiments, it was found that LF3872 and Actigen prebiotic (Alltech Inc., Nicholasville, KY, USA) exhibited synergistic anti-adhesive activity against Gram-negative pathogens. LF3872 has immunoregulatory properties: it inhibited the lipopolysaccharide-induced production of proinflammatory cytokines IL-8, IL-1β, and TNF-α in a monolayer of Caco-2 cells; inhibited the production of IL-12 and stimulated the production of IL-10 in immature human dendritic cells; and stimulated the production of TGF-β, IFN-γ, and IgA in the immunocompetent cells of intestinal Peyer's patches (PPs) in mice. These results indicate the possibility of creating a synbiotic based on LF3872 and a prebiotic derived from Saccharomyces cerevisiae cell wall components. Such innovative drugs and biologically active additives are necessary for the implementation of a strategy to reduce the spread of antibiotic-resistant strains of socially significant animal and human infections.
Databáze: MEDLINE
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