| Autor: |
Kislichkina AA; Department of Culture Collection, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Kartsev NN; Department of Molecular Microbiology, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Skryabin YP; Department of Molecular Microbiology, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Sizova AA; Department of Culture Collection, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Kanashenko ME; Department of Molecular Microbiology, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Teymurazov MG; Department of Molecular Microbiology, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Kuzina ES; Department of Training and Improvement of Specialists, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Bogun AG; Department of Culture Collection, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Fursova NK; Department of Molecular Microbiology, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Svetoch EA; Department of Molecular Microbiology, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia., Dyatlov IA; Department of Culture Collection, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia.; Department of Molecular Microbiology, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia.; Department of Training and Improvement of Specialists, State Research Center for Applied Microbiology and Biotechnology, Territory 'Kvartal A', 142279 Obolensk, Russia. |
| Abstrakt: |
Hybrid diarrheagenic E. coli strains combining genetic markers belonging to different pathotypes have emerged worldwide and have been reported as a public health concern. The most well-known hybrid strain of enteroaggregative hemorrhagic E. coli is E. coli O104:H4 strain, which was an agent of a serious outbreak of acute gastroenteritis and hemolytic uremic syndrome (HUS) in Germany in 2011. A case of intestinal infection with HUS in St. Petersburg (Russian Federation) occurred in July 2018. E. coli strain SCPM-O-B-9427 was obtained from the rectal swab of the patient with HUS. It was determined as O181:H4-, stx2- , and aggR -positive and belonged to the phylogenetic group B2. The complete genome assembly of the strain SCPM-O-B-9427 contained one chromosome and five plasmids, including the plasmid coding an aggregative adherence fimbriae I. MLST analysis showed that the strain SCPM-O-B-9427 belonged to ST678, and like E. coli O104:H4 strains, 2011C-3493 caused the German outbreak in 2011, and 2009EL-2050 was isolated in the Republic of Georgia in 2009. Comparison of three strains showed almost the same structure of their chromosomes: the plasmids pAA and the stx2a phages are very similar, but they have distinct sets of the plasmids and some unique regions in the chromosomes. |
