Screening and characterization of vaginal fluid donations for vaginal microbiota transplantation.

Autor: Yockey LJ; Department of Medicine, Massachusetts General Hospital, Boston, MA, USA., Hussain FA; Ragon Institute of MIT, MGH and Harvard, Cambridge, MA, USA.; Harvard Medical School, Boston, MA, USA., Bergerat A; Department of Medicine, Massachusetts General Hospital, Boston, MA, USA.; Department of Obstetrics and Gynecology, Massachusetts General Hospital, Boston, MA, USA., Reissis A; Ragon Institute of MIT, MGH and Harvard, Cambridge, MA, USA., Worrall D; Ragon Institute of MIT, MGH and Harvard, Cambridge, MA, USA., Xu J; Ragon Institute of MIT, MGH and Harvard, Cambridge, MA, USA., Gomez I; Ragon Institute of MIT, MGH and Harvard, Cambridge, MA, USA., Bloom SM; Ragon Institute of MIT, MGH and Harvard, Cambridge, MA, USA.; Harvard Medical School, Boston, MA, USA.; Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Mafunda NA; Ragon Institute of MIT, MGH and Harvard, Cambridge, MA, USA., Kelly J; Ragon Institute of MIT, MGH and Harvard, Cambridge, MA, USA., Kwon DS; Ragon Institute of MIT, MGH and Harvard, Cambridge, MA, USA.; Harvard Medical School, Boston, MA, USA.; Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Mitchell CM; Ragon Institute of MIT, MGH and Harvard, Cambridge, MA, USA. caroline.mitchell@mgh.harvard.edu.; Harvard Medical School, Boston, MA, USA. caroline.mitchell@mgh.harvard.edu.; Department of Obstetrics and Gynecology, Massachusetts General Hospital, Boston, MA, USA. caroline.mitchell@mgh.harvard.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Oct 26; Vol. 12 (1), pp. 17948. Date of Electronic Publication: 2022 Oct 26.
DOI: 10.1038/s41598-022-22873-y
Abstrakt: Bacterial vaginosis (BV), the overgrowth of diverse anaerobic bacteria in the vagina, is the most common cause of vaginal symptoms worldwide. BV frequently recurs after antibiotic therapy, and the best probiotic treatments only result in transient changes from BV-associated states to "optimal" communities dominated by a single species of Lactobacillus. Therefore, additional treatment strategies are needed to durably alter vaginal microbiota composition for patients with BV. Vaginal microbiota transplantation (VMT), the transfer of vaginal fluid from a healthy person with an optimal vaginal microbiota to a recipient with BV, has been proposed as one such alternative. However, VMT carries potential risks, necessitating strict safety precautions. Here, we present an FDA-approved donor screening protocol and detailed methodology for donation collection, storage, screening, and analysis of VMT material. We find that Lactobacillus viability is maintained for over six months in donated material stored at - 80 °C without glycerol or other cryoprotectants. We further show that species-specific quantitative PCR for L. crispatus and L. iners can be used as a rapid initial screening strategy to identify potential donors with optimal vaginal microbiomes. Together, this work lays the foundation for designing safe, reproducible trials of VMT as a treatment for BV.
(© 2022. The Author(s).)
Databáze: MEDLINE
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