Ultrafast one-minute electronic detection of SARS-CoV-2 infection by 3CL pro enzymatic activity in untreated saliva samples.

Autor: Borberg E; School of Chemistry, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 69978, Israel., Granot E; School of Chemistry, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 69978, Israel., Patolsky F; School of Chemistry, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 69978, Israel. fernando@post.tau.ac.il.; Department of Materials Science and Engineering, the Iby and Aladar Fleischman Faculty of Engineering, Tel Aviv University, Tel Aviv, 69978, Israel. fernando@post.tau.ac.il.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Oct 26; Vol. 13 (1), pp. 6375. Date of Electronic Publication: 2022 Oct 26.
DOI: 10.1038/s41467-022-34074-2
Abstrakt: Since its onset in December 2019, severe acute respiratory syndrome coronavirus 2, SARS-CoV-2, has caused over 6.5 million deaths worldwide as of October 2022. Attempts to curb viral transmission rely heavily on reliable testing to detect infections since a large number of transmissions are carried through asymptomatic individuals. Many available detection methods fall short in terms of reliability or point-of-care applicability. Here, we report an electrochemical approach targeting a viral proteolytic enzyme, 3CLpro, as a marker of active infection. We detect proteolytic activity directly from untreated saliva within one minute of sample incubation using a reduction-oxidation pH indicator. Importantly, clinical tests of saliva samples from 50 subjects show accurate detection of SARS-CoV-2, with high sensitivity and specificity, validated by PCR testing. These, coupled with our platform's ultrafast detection, simplicity, low cost and point-of-care compatibility, make it a promising method for the real-world SARS-CoV-2 mass-screening.
(© 2022. The Author(s).)
Databáze: MEDLINE
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