Molecular Characterization and Antifungal Susceptibility of Aspergillus spp. among Patients with Underlying Lung Diseases.

Autor:	Moglad E; Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj P.O. Box 173, Saudi Arabia.; Department of Microbiology, Medicinal and Aromatic Plants and Traditional Medicine Research Institute (MAPTMRI), National Center for Research, Khartoum P.O. Box 2404, Sudan., Saeed S; Department of Microbiology, Faculty of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum P.O. Box 407, Sudan., Saeed H; Department of Microbiology, Faculty of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum P.O. Box 407, Sudan., Ahmed H; Department of Microbiology, Faculty of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum P.O. Box 407, Sudan., Salih K; Department of Microbiology, Faculty of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum P.O. Box 407, Sudan., Altayb H; Department of Biochemistry, Faculty of Science, King Abdulaziz University, Building A 90, Jeddah P.O. Box. 80200, Saudi Arabia.; Centre for Artificial Intelligence in Precision Medicine, King Abdulaziz University, Jeddah P.O. Box. 80200, Saudi Arabia., Elhag W; Department of Basic Medical Sciences, College of Medicine, University of Bisha, Bisha P.O. Box 1290, Saudi Arabia.; Department of Microbiology, Faculty of Medical Laboratory Sciences, Al-Neelain University, Khartoum P.O. Box 12702, Sudan.
Jazyk:	angličtina
Zdroj:	Tropical medicine and infectious disease [Trop Med Infect Dis] 2022 Sep 28; Vol. 7 (10). Date of Electronic Publication: 2022 Sep 28.
DOI:	10.3390/tropicalmed7100274
Abstrakt:	Background: Pulmonary aspergillosis is a lung infection caused by Aspergillus spp., which can cause severe illnesses in immunocompromised patients with underlying lung disease or who have asthma and inhale their spores. This study aimed to screen the antifungal susceptibility of Aspergillus spp. isolated from patients with underlying pulmonary infections and characterize the isolates using PCR and sequencing. Method: Three hundred and eighty-four sputum or bronchoalveolar lavage samples were collected and processed for the isolation and identification, and characterization of Aspergillus species and molecular amplification of the ITS1-5.8S-ITS2 region by the PCR and Sanger sequencing method. Antifungal susceptibility tests for itraconazole and voriconazole were performed using the E-test. Result: The overall results revealed that out of 384 patients, 32 (8.3%) were positive for fungal growth, including 28 (87.5%) Aspergillus spp. The highest resistance rate (100 and 44.4%) was obtained from itraconazole against A. niger and A. fumigatus . In contrast, voriconazole revealed the best activities against all tested fungi compared to itraconazole. All A. flavus were sensitive to voriconazole, while only 54.5% were sensitive to itraconazole. The MICs of E-test for Aspergillus spp were 1.6 ± 1.8 and 0.6 ± 0.93 for itraconazole and voriconazole, respectively. Conclusions: The prevalence of aspergillosis was high, with a significant association with underlying lung diseases. Voriconazole was the drug of choice for isolated fungi.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu