New Amorphous Hydrogels with Proliferative Properties as Potential Tools in Wound Healing.

Autor:	Preda P; National Institute for Research and Development in Microtechnologies-IMT Bucharest, 126A Erou Iancu Nicolae, 077190 Bucharest, Romania., Enciu AM; Biochemistry-Proteomics Department, Victor Babes National Institute of Pathology, 99-101 Splaiul Inde Pendenţei, 050096 Bucharest, Romania.; Cell Biology and Histology Department, Carol Davila University of Medicine and Pharmacy, 8 Eroii Sanitari, 050474 Bucharest, Romania., Adiaconita B; National Institute for Research and Development in Microtechnologies-IMT Bucharest, 126A Erou Iancu Nicolae, 077190 Bucharest, Romania., Mihalache I; National Institute for Research and Development in Microtechnologies-IMT Bucharest, 126A Erou Iancu Nicolae, 077190 Bucharest, Romania., Craciun G; National Institute for Research and Development in Microtechnologies-IMT Bucharest, 126A Erou Iancu Nicolae, 077190 Bucharest, Romania., Boldeiu A; National Institute for Research and Development in Microtechnologies-IMT Bucharest, 126A Erou Iancu Nicolae, 077190 Bucharest, Romania., Aricov L; 'Ilie Murgulescu' Institute of Physical Chemistry, Romanian Academy, Spl. Independentei 202, 060021 Bucharest, Romania., Romanitan C; National Institute for Research and Development in Microtechnologies-IMT Bucharest, 126A Erou Iancu Nicolae, 077190 Bucharest, Romania., Stan D; DDS Diagnostic SRL, 7 Vulcan County, 031427 Bucharest, Romania.; Doctoral School of Medicine, Titu Maiorescu University, Dâmbovnicului St., 040441 Bucharest, Romania., Marculescu C; National Institute for Research and Development in Microtechnologies-IMT Bucharest, 126A Erou Iancu Nicolae, 077190 Bucharest, Romania., Tanase C; Biochemistry-Proteomics Department, Victor Babes National Institute of Pathology, 99-101 Splaiul Inde Pendenţei, 050096 Bucharest, Romania.; Faculty of Medicine, Titu Maiorescu University, Gheorghe Petrascu St., 031593 Bucharest, Romania., Avram M; National Institute for Research and Development in Microtechnologies-IMT Bucharest, 126A Erou Iancu Nicolae, 077190 Bucharest, Romania.
Jazyk:	angličtina
Zdroj:	Gels (Basel, Switzerland) [Gels] 2022 Sep 21; Vol. 8 (10). Date of Electronic Publication: 2022 Sep 21.
DOI:	10.3390/gels8100604
Abstrakt:	The study and discovery of bioactive compounds and new formulations as potential tools for promoting the repair of dermoepidermal tissue in wound healing is of continuing interest. We have developed a new formulation of amorphous hydrogel based on sodium alginate (NaAlg); type I collagen, isolated by the authors from silver carp tails (COL); glycerol (Gli); Aloe vera gel powder (AV); and silver nanoparticles obtained by green synthesis with aqueous Cinnamomum verum extract (AgNPs@CIN) and vitamin C, respectively. The gel texture of the amorphous hydrogels was achieved by the addition of Aloe vera, demonstrated by a rheological analysis. The evaluations of the cytotoxicity and cell proliferation capacity of the experimental amorphous hydrogels were performed against human foreskin fibroblast Hs27 cells (CRL-1634-ATCC). The developed gel formulations did not show a cytotoxic effect. The hydrogel variant containing AgNPs@CIN in a concentration of 8 µg Ag/gel formulation and hydrogel variant with vitamin C had proliferative activity. In addition, the antibacterial activity of the hydrogels was evaluated against S. aureus ATCC 6538, Ps. aeruginosa ATCC 27853, and E. coli ATCC 25922. The results demonstrated that the gel variant based on AgNPs@CIN in a concentration of 95 µg Ag/gel formulation and the hydrogel based on vitamin C show antibacterial activity. Therefore, the developed hydrogels with AgNPs@CIN and vitamin C could be promising alternatives in wound healing.
Databáze:	MEDLINE
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