Phylogenetic group and virulence profile classification in Escherichia coli from distinct isolation sources in Mexico.

Autor: Aguirre-Sánchez JR; Centro de Investigación en Alimentación y Desarrollo, Coordinación Regional Culiacán, Laboratorio Nacional para la Investigación en Inocuidad Alimentaria, 80110 Culiacán, Sinaloa, Mexico., Valdez-Torres JB; Centro de Investigación en Alimentación y Desarrollo, Coordinación Regional Culiacán, Laboratorio Nacional para la Investigación en Inocuidad Alimentaria, 80110 Culiacán, Sinaloa, Mexico., Del Campo NC; Facultad de Medicina Veterinaria y Zootecnia de la Universidad Autónoma de Sinaloa, 80260 Culiacán, Sinaloa, Mexico., Martínez-Urtaza J; Department of Genetics and Microbiology, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain., Del Campo NC; Centro de Investigación en Alimentación y Desarrollo, Coordinación Regional Culiacán, Laboratorio Nacional para la Investigación en Inocuidad Alimentaria, 80110 Culiacán, Sinaloa, Mexico., Lee BG; U.S. Department of Agriculture-Agricultural Research Service, Western Regional Research Center, Produce Safety and Microbiology Research Unit, Albany, CA 94710, United States., Quiñones B; U.S. Department of Agriculture-Agricultural Research Service, Western Regional Research Center, Produce Safety and Microbiology Research Unit, Albany, CA 94710, United States., Chaidez-Quiroz C; Centro de Investigación en Alimentación y Desarrollo, Coordinación Regional Culiacán, Laboratorio Nacional para la Investigación en Inocuidad Alimentaria, 80110 Culiacán, Sinaloa, Mexico. Electronic address: chaqui@ciad.mx.
Jazyk: angličtina
Zdroj: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2022 Dec; Vol. 106, pp. 105380. Date of Electronic Publication: 2022 Oct 22.
DOI: 10.1016/j.meegid.2022.105380
Abstrakt: Escherichia coli is a leading cause of human enteric diseases worldwide. The rapid and accurate causal agent identification to a particular source represents a crucial step in the establishment of safety and health measures in the affected human populations and would thus provide insights into the relationship of traits that may contribute for pathogen persistence in a particular reservoir. The objective of the present study was to characterize over two hundred E. coli strains from different isolation sources in Mexico by conducting a correspondence analysis to explore associations with the detected phylogenetic groups. The results indicated that E. coli strains, recovered from distinct sources in Mexico, were classified into phylogroups B1 (35.8%), A (27.8%), and D (12.3%) and were clustered to particular clades according to the predicted phylogroups. The results from correspondence analysis showed that E. coli populations from distinct sources in Mexico, belonging to different phylogroups, were not dispersed randomly and were associated with a particular isolation source. Phylogroup A was strongly associated with human sources, and the phylogroup B1 showed a significant relationship with food sources. Additionally, phylogroup D was also related to human sources. Phylogroup B2 was associated with herbivorous and omnivorous mammals. Moreover, common virulence genes in the examined E. coli strains, assigned to all phylogroups, were identified as essential markers for survival and invasion in the host. Although virulence profiles varied among the detected phylogroups, E. coli strains belonging to phylogroup D, associated with humans, were found to contain the largest virulence gene repertoire conferring for persistence and survival in the host. In summary, these findings provide fundamental information for a better characterization of pathogenic E. coli, recovered from distinct isolation sources in Mexico and would assist in the development of better tools for identifying potential transmission routes of contamination.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Published by Elsevier B.V.)
Databáze: MEDLINE
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