Bovine and human endometrium-derived hydrogels support organoid culture from healthy and cancerous tissues.

Autor: Jamaluddin MFB; Global Centre for Gynecological Diseases, The University of Newcastle, Australia, Callaghan, NSW 2308, Australia.; School of Biomedical Sciences and Pharmacy, The University of Newcastle, Australia, Callaghan, NSW 2308, Australia.; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia., Ghosh A; Global Centre for Gynecological Diseases, The University of Newcastle, Australia, Callaghan, NSW 2308, Australia.; School of Biomedical Sciences and Pharmacy, The University of Newcastle, Australia, Callaghan, NSW 2308, Australia.; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia., Ingle A; Ian Potter NanoBioSensing Facility, NanoBiotechnology Research Laboratory, School of Science, RMIT University, Melbourne, VIC 3000, Australia., Mohammed R; Global Centre for Gynecological Diseases, The University of Newcastle, Australia, Callaghan, NSW 2308, Australia.; School of Biomedical Sciences and Pharmacy, The University of Newcastle, Australia, Callaghan, NSW 2308, Australia.; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia., Ali A; School of Biomedical Sciences and Pharmacy, The University of Newcastle, Australia, Callaghan, NSW 2308, Australia.; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia., Bahrami M; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia.; School of Environmental and Life Sciences, The University of Newcastle, Australia, Callaghan, NSW 2308, Australia., Kaiko G; School of Biomedical Sciences and Pharmacy, The University of Newcastle, Australia, Callaghan, NSW 2308, Australia.; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia., Gibb Z; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia.; School of Environmental and Life Sciences, The University of Newcastle, Australia, Callaghan, NSW 2308, Australia., Filipe EC; The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Medicine and Health, University of New South Wales (UNSW), Sydney, NSW 2052, Australia.; School of Clinical Medicine, St. Vincent's Clinical Campus, Medicine and Health, UNSW, Sydney, NSW 2052, Australia., Cox TR; The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Medicine and Health, University of New South Wales (UNSW), Sydney, NSW 2052, Australia.; School of Clinical Medicine, St. Vincent's Clinical Campus, Medicine and Health, UNSW, Sydney, NSW 2052, Australia., Boulton A; Hunter New England Centre for Gynecological Cancer, John Hunter Hospital, Newcastle, NSW 2305, Australia., O'Sullivan R; Hunter New England Centre for Gynecological Cancer, John Hunter Hospital, Newcastle, NSW 2305, Australia., Ius Y; Hunter New England Centre for Gynecological Cancer, John Hunter Hospital, Newcastle, NSW 2305, Australia., Karakoti A; Global Innovative Centre for Advanced Nanomaterials, College of Engineering, Science, and Environment, The University of Newcastle, Australia, Callaghan, NSW 2308, Australia., Vinu A; Global Innovative Centre for Advanced Nanomaterials, College of Engineering, Science, and Environment, The University of Newcastle, Australia, Callaghan, NSW 2308, Australia., Nahar P; Department of Maternity and Gynecology, John Hunter Hospital, Newcastle, NSW 2305, Australia., Jaaback K; Hunter New England Centre for Gynecological Cancer, John Hunter Hospital, Newcastle, NSW 2305, Australia., Bansal V; Ian Potter NanoBioSensing Facility, NanoBiotechnology Research Laboratory, School of Science, RMIT University, Melbourne, VIC 3000, Australia., Tanwar PS; Global Centre for Gynecological Diseases, The University of Newcastle, Australia, Callaghan, NSW 2308, Australia.; School of Biomedical Sciences and Pharmacy, The University of Newcastle, Australia, Callaghan, NSW 2308, Australia.; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia.
Jazyk: angličtina
Zdroj: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2022 Nov; Vol. 119 (44), pp. e2208040119. Date of Electronic Publication: 2022 Oct 24.
DOI: 10.1073/pnas.2208040119
Abstrakt: Organoid technology has provided unique insights into human organ development, function, and diseases. Patient-derived organoids are increasingly used for drug screening, modeling rare disorders, designing regenerative therapies, and understanding disease pathogenesis. However, the use of Matrigel to grow organoids represents a major challenge in the clinical translation of organoid technology. Matrigel is a poorly defined mixture of extracellular matrix proteins and growth factors extracted from the Engelbreth-Holm-Swarm mouse tumor. The extracellular matrix is a major driver of multiple cellular processes and differs significantly between tissues as well as in healthy and disease states of the same tissue. Therefore, we envisioned that the extracellular matrix derived from a native healthy tissue would be able to support organoid growth akin to organogenesis in vivo. Here, we have developed hydrogels from decellularized human and bovine endometrium. These hydrogels supported the growth of mouse and human endometrial organoids, which was comparable to Matrigel. Organoids grown in endometrial hydrogels were proteomically more similar to the native tissue than those cultured in Matrigel. Proteomic and Raman microspectroscopy analyses showed that the method of decellularization affects the biochemical composition of hydrogels and, subsequently, their ability to support organoid growth. The amount of laminin in hydrogels correlated with the number and shape of organoids. We also demonstrated the utility of endometrial hydrogels in developing solid scaffolds for supporting high-throughput, cell culture-based applications. In summary, endometrial hydrogels overcome a major limitation of organoid technology and greatly expand the applicability of organoids to understand endometrial biology and associated pathologies.
Databáze: MEDLINE
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